Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS30085 and AZOBR_RS30090 are separated by 75 nucleotidesAZOBR_RS30090 and AZOBR_RS30095 are separated by 129 nucleotidesAZOBR_RS30095 and AZOBR_RS30105 are separated by 1964 nucleotidesAZOBR_RS30105 and AZOBR_RS30120 are separated by 2855 nucleotides AZOBR_RS30085: AZOBR_RS30085 - DNA-binding protein, at 89,607 to 90,215 _RS30085 AZOBR_RS30090: AZOBR_RS30090 - hypothetical protein, at 90,291 to 90,614 _RS30090 AZOBR_RS30095: AZOBR_RS30095 - hypothetical protein, at 90,744 to 91,787 _RS30095 AZOBR_RS30105: AZOBR_RS30105 - hypothetical protein, at 93,752 to 94,092 _RS30105 AZOBR_RS30120: AZOBR_RS30120 - C4-dicarboxylate ABC transporter permease, at 96,948 to 98,231 _RS30120
Group Condition AZOBR_RS30085 AZOBR_RS30090 AZOBR_RS30095 AZOBR_RS30105 AZOBR_RS30120
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 N.D. -4.4 N.D. -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 N.D. -4.4 N.D. -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 N.D. -4.3 N.D. -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 N.D. -2.9 N.D. -0.2
nitrogen source Ammonium chloride (N) -0.0 N.D. -2.4 N.D. +0.0
motility outer cut, LB soft agar motility assay -1.3 N.D. -0.9 N.D. +0.0
carbon source D-Gluconic Acid (C) -0.0 N.D. -1.9 N.D. -0.0
stress Paraquat dichloride 0.04 mg/ml -0.6 N.D. -1.3 N.D. +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 N.D. -1.9 N.D. +0.3
stress D-Cycloserine 0.125 mg/ml +0.3 N.D. -1.9 N.D. -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 N.D. -1.8 N.D. +0.0
stress 2-pentyl-furan 2.671 mM +0.2 N.D. -1.6 N.D. +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.5 N.D. -1.3 N.D. -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.7 N.D. -1.5 N.D. -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 N.D. -1.1 N.D. -0.1
nitrogen source L-Histidine (N) +0.4 N.D. -1.1 N.D. +0.3
carbon source a-Ketoglutaric (C) +0.8 N.D. -1.2 N.D. +0.1
stress Chlorite 1 mM +0.6 N.D. -0.9 N.D. -0.1
nitrogen source L-Isoleucine (N) +1.0 N.D. -0.6 N.D. -0.3
carbon source a-Ketoglutaric (C) +0.9 N.D. -0.8 N.D. -0.0
stress Cobalt chloride 0.2 mM +1.0 N.D. -0.4 N.D. -0.2
stress Cobalt chloride 0.3 mM +1.4 N.D. +0.2 N.D. -0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.6 N.D. +1.1 N.D. -0.1
motility inner cut, LB soft agar motility assay +0.2 N.D. +1.3 N.D. +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.1 N.D. +1.6 N.D. +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.0 N.D. +1.8 N.D. +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.4 N.D. +1.7 N.D. -0.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.1 N.D. +2.1 N.D. -0.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.3 N.D. +2.5 N.D. -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.9 N.D. +2.2 N.D. +0.0
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AZOBR_RS30090
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