Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS30080 and AZOBR_RS30085 are separated by 309 nucleotidesAZOBR_RS30085 and AZOBR_RS30090 are separated by 75 nucleotidesAZOBR_RS30090 and AZOBR_RS30095 are separated by 129 nucleotidesAZOBR_RS30095 and AZOBR_RS30105 are separated by 1964 nucleotides AZOBR_RS30080: AZOBR_RS30080 - flagellar hook protein, at 88,065 to 89,297 _RS30080 AZOBR_RS30085: AZOBR_RS30085 - DNA-binding protein, at 89,607 to 90,215 _RS30085 AZOBR_RS30090: AZOBR_RS30090 - hypothetical protein, at 90,291 to 90,614 _RS30090 AZOBR_RS30095: AZOBR_RS30095 - hypothetical protein, at 90,744 to 91,787 _RS30095 AZOBR_RS30105: AZOBR_RS30105 - hypothetical protein, at 93,752 to 94,092 _RS30105
Group Condition AZOBR_RS30080 AZOBR_RS30085 AZOBR_RS30090 AZOBR_RS30095 AZOBR_RS30105
motility outer cut, LB soft agar motility assay -2.9 -1.3 N.D. -0.9 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.2 N.D. -4.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.1 N.D. -4.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.3 N.D. -4.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.1 N.D. -2.9 N.D.
nitrogen source Ammonium chloride (N) +0.1 -0.0 N.D. -2.4 N.D.
carbon source D-Gluconic Acid (C) -0.1 -0.0 N.D. -1.9 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.1 N.D. -1.9 N.D.
stress Paraquat dichloride 0.04 mg/ml -0.0 -0.6 N.D. -1.3 N.D.
stress D-Cycloserine 0.125 mg/ml -0.1 +0.3 N.D. -1.9 N.D.
stress 2-pentyl-furan 2.671 mM -0.3 +0.2 N.D. -1.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.5 N.D. -1.8 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.0 +0.7 N.D. -1.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.1 +0.5 N.D. -1.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.5 N.D. -1.1 N.D.
stress Chlorite 1 mM -0.1 +0.6 N.D. -0.9 N.D.
nitrogen source L-Histidine (N) +0.3 +0.4 N.D. -1.1 N.D.
carbon source a-Ketoglutaric (C) +0.2 +0.8 N.D. -1.2 N.D.
nitrogen source L-Isoleucine (N) -0.4 +1.0 N.D. -0.6 N.D.
stress Cobalt chloride 0.2 mM -0.3 +1.0 N.D. -0.4 N.D.
lb LB -0.2 +0.6 N.D. +0.8 N.D.
stress Cobalt chloride 0.3 mM -0.2 +1.4 N.D. +0.2 N.D.
motility inner cut, LB soft agar motility assay +0.2 +0.2 N.D. +1.3 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.0 +0.6 N.D. +1.1 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.4 -0.0 N.D. +1.8 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.4 +0.1 N.D. +1.6 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.3 +0.4 N.D. +1.7 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.4 +0.1 N.D. +2.1 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.5 +0.3 N.D. +2.5 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.5 +0.9 N.D. +2.2 N.D.
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