Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS29975 and AZOBR_RS29980 are separated by 132 nucleotidesAZOBR_RS29980 and AZOBR_RS29985 are separated by 30 nucleotidesAZOBR_RS29985 and AZOBR_RS29990 are separated by 303 nucleotidesAZOBR_RS29990 and AZOBR_RS29995 are separated by 28 nucleotides AZOBR_RS29975: AZOBR_RS29975 - AsnC family transcriptional regulator, at 65,722 to 66,150 _RS29975 AZOBR_RS29980: AZOBR_RS29980 - saccharopine dehydrogenase, at 66,283 to 67,410 _RS29980 AZOBR_RS29985: AZOBR_RS29985 - pyruvate oxidase, at 67,441 to 69,195 _RS29985 AZOBR_RS29990: AZOBR_RS29990 - putative dioxygenase subunit, at 69,499 to 70,425 _RS29990 AZOBR_RS29995: AZOBR_RS29995 - PAS sensor protein, at 70,454 to 70,879 _RS29995
Group Condition AZOBR_RS29975 AZOBR_RS29980 AZOBR_RS29985 AZOBR_RS29990 AZOBR_RS29995
nitrogen source L-Lysine (N) N.D. -4.3 -0.2 -0.3 +0.2
nitrogen source L-Lysine (N) N.D. -3.8 -0.1 -0.0 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -2.8 +0.0 -1.0 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -1.6 +0.1 -0.8 +0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -1.0 +0.1 -1.0 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.2 +0.2 -0.4 -0.6
anaerobic; rich media LB; anaerobic N.D. -2.3 -0.0 +0.1 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. -1.5 -0.0 -0.3 +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -1.0 -0.1 -0.7 +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.9 -0.1 -1.2 +0.5
stress Paraquat dichloride 0.04 mg/ml N.D. -0.3 -0.2 -0.9 -0.1
lb LB N.D. -0.3 -0.2 -0.7 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 -0.2 -1.1 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. -1.3 -0.1 -0.0 +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. -0.6 +0.0 -0.6 -0.1
carbon source L-Proline (C) N.D. -0.7 -0.1 -0.8 +0.3
stress 2-pentyl-furan 1.780666667 mM N.D. -0.6 +0.0 +0.1 -0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. -0.8 -0.1 -0.4 +0.2
stress Sisomicin 0.01 mg/ml N.D. +0.0 +0.3 -0.3 -1.0
carbon source D-Gluconic Acid (C) N.D. -0.8 -0.3 -0.3 +0.7
stress 3-octanone 6.3476 mM N.D. -0.9 +0.2 +0.5 -0.4
stress 3-octanone 4.2317 mM N.D. -0.8 -0.1 +0.1 +0.4
motility outer cut, LB soft agar motility assay N.D. -0.0 +0.3 -1.0 +0.4
nitrogen source L-Isoleucine (N) N.D. -0.1 -0.1 +0.7 -0.7
stress 2-pentyl-furan 1.335528 mM N.D. +0.5 -0.2 +0.2 -0.5
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. -0.6 -0.0 +0.5 +0.5
nitrogen source casaminos (N) N.D. -0.1 +0.1 -0.4 +0.7
stress 2-Furfuraldehyde 0.2 vol% N.D. +0.6 +0.1 -0.5 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.2 -0.0 +0.5 +0.6
stress 3-octanone 6.3476 mM N.D. -0.6 -0.0 +0.6 +0.9
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