Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS29920 and AZOBR_RS29925 overlap by 4 nucleotidesAZOBR_RS29925 and AZOBR_RS29930 are separated by 125 nucleotidesAZOBR_RS29930 and AZOBR_RS29935 are separated by 20 nucleotidesAZOBR_RS29935 and AZOBR_RS29940 are separated by 13 nucleotides AZOBR_RS29920: AZOBR_RS29920 - conserved exported protein of unknown function, at 56,514 to 57,122 _RS29920 AZOBR_RS29925: AZOBR_RS29925 - ATP synthase, at 57,119 to 58,459 _RS29925 AZOBR_RS29930: AZOBR_RS29930 - flagellar basal body rod protein FlgG, at 58,585 to 58,977 _RS29930 AZOBR_RS29935: AZOBR_RS29935 - flagellar basal-body rod protein FlgC, at 58,998 to 59,417 _RS29935 AZOBR_RS29940: AZOBR_RS29940 - flagellar basal body protein FliE, at 59,431 to 59,793 _RS29940
Group Condition AZOBR_RS29920 AZOBR_RS29925 AZOBR_RS29930 AZOBR_RS29935 AZOBR_RS29940
motility outer cut, LB soft agar motility assay N.D. -2.5 -2.2 -3.2 N.D.
nitrogen source L-Isoleucine (N) N.D. -0.4 -4.1 -3.0 N.D.
stress Cobalt chloride 0.3 mM N.D. -0.2 -1.5 -1.3 N.D.
nitrogen source casaminos (N) N.D. +0.1 +0.6 -3.2 N.D.
nitrogen source Parabanic Acid (N) N.D. -0.4 -0.8 -0.9 N.D.
stress 1-octen-3-ol 0.6784116667 mM N.D. -0.3 -1.1 -0.1 N.D.
nitrogen source L-Leucine (N) N.D. -0.4 -1.0 +0.4 N.D.
nitrogen source L-Threonine (N) N.D. +0.3 +0.5 -1.4 N.D.
stress 2-pentyl-furan 1.335528 mM N.D. -0.5 +1.1 -0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.0 -0.9 +0.7 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.1 -1.4 +1.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.4 +0.9 -0.5 N.D.
stress 2-pentyl-furan 2.671 mM N.D. -0.3 +0.9 -0.5 N.D.
stress 1-octen-3-ol 0.7753276667 mM N.D. +0.4 +0.7 -0.7 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.1 -0.6 +0.9 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.0 -0.6 +1.3 N.D.
nitrogen source L-Threonine (N) N.D. -0.2 -0.3 +1.2 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.2 -0.7 +1.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.4 +1.0 +0.3 N.D.
motility inner cut, LB soft agar motility assay N.D. +0.4 +1.1 -0.3 N.D.
nitrogen source L-Glutamic (N) N.D. -0.2 +0.5 +1.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.2 +0.2 +1.4 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.4 -0.4 +1.4 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.3 +0.0 +1.4 N.D.
motility inner cut, LB soft agar motility assay N.D. -0.0 +1.4 +0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.2 +1.4 +0.6 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.5 +0.1 +1.3 N.D.
nitrogen source Ammonium chloride (N) N.D. +0.1 +0.8 +1.3 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.3 +0.8 +1.2 N.D.
carbon source D-Gluconic Acid (C) N.D. -0.1 +1.0 +2.2 N.D.
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