Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS29800 and AZOBR_RS29805 are separated by 4 nucleotidesAZOBR_RS29805 and AZOBR_RS29810 are separated by 102 nucleotidesAZOBR_RS29810 and AZOBR_RS29815 are separated by 97 nucleotidesAZOBR_RS29815 and AZOBR_RS29820 are separated by 339 nucleotides AZOBR_RS29800: AZOBR_RS29800 - hypothetical protein, at 35,326 to 35,916 _RS29800 AZOBR_RS29805: AZOBR_RS29805 - hypothetical protein, at 35,921 to 36,160 _RS29805 AZOBR_RS29810: AZOBR_RS29810 - phosphoribosylglycinamide formyltransferase, at 36,263 to 37,432 _RS29810 AZOBR_RS29815: AZOBR_RS29815 - conserved exported protein of unknown function, at 37,530 to 38,027 _RS29815 AZOBR_RS29820: AZOBR_RS29820 - ABC transporter substrate-binding protein, at 38,367 to 39,398 _RS29820
Group Condition AZOBR_RS29800 AZOBR_RS29805 AZOBR_RS29810 AZOBR_RS29815 AZOBR_RS29820
motility outer cut, LB soft agar motility assay +0.1 N.D. -0.1 -2.0 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 N.D. -0.3 -1.3 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 N.D. -0.5 -0.9 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 N.D. -0.3 -0.0 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 N.D. +0.1 -0.8 -0.4
stress Cobalt chloride 0.3 mM -0.3 N.D. -0.2 -1.1 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 N.D. -0.7 -1.3 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. -0.6 -0.6 -0.2
motility inner cut, LB soft agar motility assay -0.7 N.D. +0.6 -0.5 -0.1
stress Cobalt chloride 0.2 mM -0.2 N.D. -0.7 +0.2 -0.1
carbon source D-Lactate (C) +0.1 N.D. -0.6 -0.3 +0.1
stress 2-pentyl-furan 2.671 mM +0.4 N.D. -0.0 -0.5 -0.2
stress Sisomicin 0.01 mg/ml +0.3 N.D. -0.7 +0.3 -0.4
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.2 N.D. +0.2 -0.6 -0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.2 N.D. +0.2 -0.6 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 N.D. -0.0 +0.8 -0.1
carbon source D-Gluconic Acid (C) -0.5 N.D. -0.0 +0.3 +0.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.1 N.D. +0.3 -0.6 +0.5
motility inner cut, LB soft agar motility assay +0.1 N.D. +0.6 -0.7 +0.2
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.4 N.D. +0.5 -0.4 +0.1
stress 2-pentyl-furan 1.335528 mM -0.2 N.D. +0.5 +0.4 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.3 N.D. +0.4 -0.1 +0.3
stress 2-pentyl-furan 1.335528 mM +0.2 N.D. -0.1 +0.4 +0.4
stress 3-octanone 4.2317 mM -0.1 N.D. +0.4 +0.4 +0.2
stress 2-pentyl-furan 1.780666667 mM +0.2 N.D. +0.1 +0.8 -0.2
stress 3-octanone 6.3476 mM -0.1 N.D. +0.7 +0.3 +0.1
stress 3-octanone 6.3476 mM +0.1 N.D. +0.4 +0.5 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.1 N.D. +0.5 +0.6 +0.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.2 N.D. +0.7 +0.5 +0.0
stress 2-pentyl-furan 1.780666667 mM +0.1 N.D. +0.6 +0.8 -0.0
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