Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS29570 and AZOBR_RS29580 are separated by 7432 nucleotidesAZOBR_RS29580 and AZOBR_RS29585 are separated by 920 nucleotidesAZOBR_RS29585 and AZOBR_RS29590 are separated by 30 nucleotidesAZOBR_RS29590 and AZOBR_RS29595 overlap by 11 nucleotides AZOBR_RS29570: AZOBR_RS29570 - antibiotic synthesis protein MbtH, at 758,548 to 758,754 _RS29570 AZOBR_RS29580: AZOBR_RS29580 - hypothetical protein, at 766,187 to 767,638 _RS29580 AZOBR_RS29585: AZOBR_RS29585 - hypothetical protein, at 768,559 to 768,846 _RS29585 AZOBR_RS29590: AZOBR_RS29590 - hypothetical protein, at 768,877 to 769,620 _RS29590 AZOBR_RS29595: AZOBR_RS29595 - hypothetical protein, at 769,610 to 770,242 _RS29595
Group Condition AZOBR_RS29570 AZOBR_RS29580 AZOBR_RS29585 AZOBR_RS29590 AZOBR_RS29595
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.3 -3.6 -0.3 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.4 -2.9 -0.2 -0.1
stress Nickel (II) chloride 0.5 mM N.D. -0.2 -2.4 -0.3 -0.1
stress Benzethonium chloride 0.003 mM N.D. +0.1 -2.2 -0.4 -0.1
stress D-Cycloserine 0.125 mg/ml N.D. +0.3 -2.8 -0.0 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.4 -1.2 +0.3 -0.5
nitrogen source L-Leucine (N) N.D. -0.3 -1.3 -0.1 -0.1
nitrogen source L-Glutamic (N) N.D. -0.4 -1.3 +0.1 -0.2
stress 2-pentyl-furan 1.780666667 mM N.D. +0.4 -1.5 -0.3 -0.2
carbon source a-Ketoglutaric (C) N.D. -0.4 -0.6 -0.4 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.6 -0.1 -0.6 -0.2
carbon source Glycerol (C) N.D. -0.2 -1.3 +0.2 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.2 -0.8 +0.3 -0.5
motility inner cut, LB soft agar motility assay N.D. +0.4 -1.0 -0.1 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.6 -0.2 -0.2 -0.2
stress nitrate 100 mM N.D. -0.1 -0.8 -0.4 +0.0
lb LB N.D. +0.1 -0.9 -0.2 -0.2
stress Cobalt chloride 0.3 mM N.D. +0.1 -1.5 +0.2 +0.4
lb no stress control LB no stress control N.D. -0.2 -0.6 -0.2 +0.2
stress 3-octanone 4.2317 mM N.D. -0.5 -0.6 +0.3 +0.1
nitrogen source Urea (N) N.D. -0.3 +0.5 -0.4 -0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.3 -0.8 +0.2 +0.0
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.4 -0.7 -0.3 +0.3
motility outer cut, LB soft agar motility assay N.D. +0.5 -0.4 -0.2 +0.6
stress 2-pentyl-furan 1.780666667 mM N.D. +0.5 +0.2 -0.2 +0.5
stress Sisomicin 0.01 mg/ml N.D. +0.0 +1.0 +0.2 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.3 +1.1 -0.1 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.2 +0.9 +0.0 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.4 +1.2 +0.1 -0.2
stress 3-octanone 6.3476 mM N.D. +0.2 +0.7 +0.4 +0.2
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