Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS28255 and AZOBR_RS28260 are separated by 22 nucleotidesAZOBR_RS28260 and AZOBR_RS28270 are separated by 1100 nucleotidesAZOBR_RS28270 and AZOBR_RS28280 are separated by 827 nucleotidesAZOBR_RS28280 and AZOBR_RS28285 overlap by 4 nucleotides AZOBR_RS28255: AZOBR_RS28255 - hypothetical protein, at 508,572 to 509,240 _RS28255 AZOBR_RS28260: AZOBR_RS28260 - putative Sec-independent protein translocase protein TatA/E (fragment), at 509,263 to 509,451 _RS28260 AZOBR_RS28270: AZOBR_RS28270 - copper chaperone, at 510,552 to 511,151 _RS28270 AZOBR_RS28280: AZOBR_RS28280 - ABC transporter ATP-binding protein, at 511,979 to 512,848 _RS28280 AZOBR_RS28285: AZOBR_RS28285 - carbohydrate-binding protein, at 512,845 to 514,212 _RS28285
Group Condition AZOBR_RS28255 AZOBR_RS28260 AZOBR_RS28270 AZOBR_RS28280 AZOBR_RS28285
motility inner cut, LB soft agar motility assay -4.2 N.D. -0.0 -0.3 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.9 N.D. -0.2 -0.3 -0.6
lb LB -2.8 N.D. +0.6 -0.1 -0.0
carbon source L-Lactate (C) -1.6 N.D. -0.2 -0.2 -0.0
stress Chlorite 1 mM -0.5 N.D. -0.3 -0.6 -0.2
stress Benzethonium chloride 0.003 mM -0.8 N.D. -0.3 -0.6 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.4 N.D. +0.2 -0.4 -0.2
lb LB -1.6 N.D. -0.0 -0.1 +0.0
stress Thallium(I) acetate 0.008 mg/ml -0.1 N.D. -0.7 -0.5 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.9 N.D. +0.4 -0.3 +0.4
stress 2-pentyl-furan 1.780666667 mM +0.7 N.D. -0.6 -0.8 -0.4
motility inner cut, LB soft agar motility assay -1.4 N.D. +0.1 +0.3 +0.2
stress Cobalt chloride 0.2 mM +0.7 N.D. -0.6 -0.6 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.8 N.D. -0.5 -0.6 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.1 N.D. +0.3 +0.5 +0.3
carbon source D,L-Lactate (C) -0.6 N.D. +0.3 +0.4 +0.2
motility inner cut, LB soft agar motility assay +1.1 N.D. +0.0 -0.4 +0.1
nitrogen source L-Phenylalanine (N) +0.7 N.D. +0.3 +0.5 +0.1
nitrogen source casaminos (N) +0.0 N.D. +0.6 +0.7 +0.3
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.1 N.D. +0.4 +0.7 +0.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.5 N.D. +0.5 +0.4 +0.2
nitrogen source Ammonium chloride (N) +0.4 N.D. +0.4 +0.5 +0.5
nitrogen source casaminos (N) +0.6 N.D. +0.5 +0.4 +0.2
nitrogen source L-Lysine (N) +0.6 N.D. +0.4 +0.5 +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.5 N.D. +0.5 +0.4 +0.3
carbon source D-Lactate (C) +0.7 N.D. +0.3 +0.4 +0.3
nitrogen source Cytosine (N) +0.7 N.D. +0.4 +0.5 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.4 N.D. +0.3 +0.7 +0.4
carbon source butyrate (C) +0.6 N.D. +0.5 +0.6 +0.2
nitrogen source L-Threonine (N) +1.4 N.D. +0.5 +0.6 +0.4
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