Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS28070 and AZOBR_RS28075 are separated by 7 nucleotidesAZOBR_RS28075 and AZOBR_RS28080 are separated by 121 nucleotidesAZOBR_RS28080 and AZOBR_RS28095 are separated by 7294 nucleotidesAZOBR_RS28095 and AZOBR_RS28100 are separated by 298 nucleotides AZOBR_RS28070: AZOBR_RS28070 - hypothetical protein, at 464,832 to 465,290 _RS28070 AZOBR_RS28075: AZOBR_RS28075 - membrane protein, at 465,298 to 466,434 _RS28075 AZOBR_RS28080: AZOBR_RS28080 - GntR family transcriptional regulator, at 466,556 to 467,281 _RS28080 AZOBR_RS28095: AZOBR_RS28095 - transporter, at 474,576 to 475,436 _RS28095 AZOBR_RS28100: AZOBR_RS28100 - LysR family transcriptional regulator, at 475,735 to 476,625 _RS28100
Group Condition AZOBR_RS28070 AZOBR_RS28075 AZOBR_RS28080 AZOBR_RS28095 AZOBR_RS28100
stress D-Cycloserine 0.125 mg/ml -0.4 +0.1 N.D. -4.9 -0.1
stress 2-pentyl-furan 1.335528 mM -0.2 +0.1 N.D. -4.0 -0.4
lb no stress control LB no stress control +0.2 -0.1 N.D. -2.9 -0.5
motility inner cut, LB soft agar motility assay +0.1 -0.1 N.D. -2.5 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.4 -0.0 N.D. -0.5 -0.1
stress 2-pentyl-furan 2.671 mM -0.5 +0.2 N.D. -2.0 -0.4
nitrogen source casaminos (N) -1.3 -0.1 N.D. -1.2 -0.1
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM -0.2 -0.0 N.D. -2.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.8 -0.2 N.D. -0.4 -0.3
stress 2-pentyl-furan 1.335528 mM +0.8 -0.1 N.D. -2.9 -0.5
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM -0.7 +0.0 N.D. -2.2 +0.5
stress 3-octanone 5.2896 mM -0.4 -0.5 N.D. -0.7 -0.6
stress 2-pentyl-furan 1.780666667 mM -0.3 -0.0 N.D. -1.8 +0.0
motility inner cut, LB soft agar motility assay -0.5 -0.3 N.D. -1.6 +0.5
stress 3-octanone 4.2317 mM +0.2 +0.0 N.D. -1.4 -0.5
stress Paraquat dichloride 0.04 mg/ml +0.3 -0.2 N.D. -1.0 -0.5
stress 2-pentyl-furan 1.335528 mM +0.3 +0.2 N.D. -1.2 -0.6
stress 3-octanone 6.3476 mM -0.4 +0.4 N.D. -0.8 -0.5
stress 2-pentyl-furan 1.780666667 mM +0.8 -0.1 N.D. -0.4 -1.1
stress 2-pentyl-furan 1.335528 mM +0.9 -0.0 N.D. -1.1 -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.9 -0.1 N.D. +0.8 -0.4
carbon source D-Fructose (C) +0.7 -0.0 N.D. -1.3 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 +0.2 N.D. +0.8 -0.3
stress Cobalt chloride 0.3 mM +0.8 -0.1 N.D. -0.7 -0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -1.0 +0.2 N.D. +0.6 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.0 +0.4 N.D. +0.5 +0.2
stress 2-pentyl-furan 1.780666667 mM -1.5 +0.5 N.D. +1.4 -0.2
motility outer cut, LB soft agar motility assay +1.4 -0.0 N.D. -0.4 -0.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.2 +0.4 N.D. +0.7 +0.5
stress Sisomicin 0.01 mg/ml +1.5 -0.0 N.D. +1.5 -1.0
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