Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS26660 and AZOBR_RS26665 are separated by 40 nucleotidesAZOBR_RS26665 and AZOBR_RS26670 are separated by 192 nucleotidesAZOBR_RS26670 and AZOBR_RS26675 are separated by 5 nucleotidesAZOBR_RS26675 and AZOBR_RS26680 are separated by 85 nucleotides AZOBR_RS26660: AZOBR_RS26660 - signal peptidase II, at 129,467 to 129,997 _RS26660 AZOBR_RS26665: AZOBR_RS26665 - conserved hypothetical protein; putative signal peptide; twin-arginine translocation signal domain protein, at 130,038 to 130,688 _RS26665 AZOBR_RS26670: AZOBR_RS26670 - zinc transporter ZitB, at 130,881 to 131,759 _RS26670 AZOBR_RS26675: AZOBR_RS26675 - IclR family transcriptional regulator, at 131,765 to 132,649 _RS26675 AZOBR_RS26680: AZOBR_RS26680 - ABC transporter permease, at 132,735 to 133,655 _RS26680
Group Condition AZOBR_RS26660 AZOBR_RS26665 AZOBR_RS26670 AZOBR_RS26675 AZOBR_RS26680
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -2.5 -4.4 -4.4 -0.5 -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -3.7 -2.4 -4.7 -0.7 -0.0
stress Cobalt chloride 0.2 mM -5.2 -3.0 -2.0 -0.5 -0.1
stress Cobalt chloride 0.3 mM -1.7 -2.3 -3.1 -0.5 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -1.5 -3.1 +0.2 -1.3 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -4.9 +0.7 +0.3 -1.1 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -4.8 -0.9 +0.5 +0.4 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -3.1 +0.2 -0.1 -1.2 +0.3
stress 3-octanone 6.3476 mM -2.0 -1.7 -0.2 +0.1 +0.2
stress Chlorite 1 mM -0.4 -1.1 -0.4 -1.0 -0.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -1.9 +0.8 +0.1 -1.6 -0.2
stress copper (II) chloride 2 mM -1.0 -0.1 -1.2 -0.0 -0.2
lb no stress control LB no stress control -0.7 -0.8 -0.6 -0.0 -0.3
stress Paraquat dichloride 0.02 mg/ml +0.2 -1.6 -0.2 -0.0 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.3 -0.5 +0.3 -2.0 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.6 -0.4 -0.1 +0.0 +0.6
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.3 +0.7 -0.1 -2.3 +0.1
stress Sisomicin 0.01 mg/ml +0.4 +0.2 +0.1 -1.5 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -1.5 +0.5 +0.7 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.4 -0.7 +0.6 -0.6
lb LB +0.5 +0.8 -0.6 -1.2 -0.3
anaerobic; rich media LB; anaerobic +0.8 -0.2 +0.1 -1.0 -0.2
lb LB -0.2 +1.1 -0.2 -1.1 -0.1
stress Paraquat dichloride 0.04 mg/ml +0.4 +0.6 -0.0 -1.3 +0.1
motility inner cut, LB soft agar motility assay +1.1 -2.0 +0.2 +0.1 +0.3
nitrogen source L-Glutamic (N) -1.2 +1.2 +0.1 -0.3 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.1 -0.4 +0.5 +0.6 +0.3
stress Benzethonium chloride 0.003 mM +1.4 +0.0 +0.2 -1.0 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.2 +1.4 -0.1 +0.2 -0.8
stress 3-octanone 6.3476 mM +1.5 +1.1 -0.4 -0.0 +0.3
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