Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS26590 and AZOBR_RS26595 are separated by 110 nucleotidesAZOBR_RS26595 and AZOBR_RS26600 overlap by 4 nucleotidesAZOBR_RS26600 and AZOBR_RS26605 overlap by 4 nucleotidesAZOBR_RS26605 and AZOBR_RS26610 are separated by 159 nucleotides AZOBR_RS26590: AZOBR_RS26590 - AraC family transcriptional regulator, at 109,831 to 110,640 _RS26590 AZOBR_RS26595: AZOBR_RS26595 - membrane protein, at 110,751 to 112,298 _RS26595 AZOBR_RS26600: AZOBR_RS26600 - membrane protein, at 112,295 to 112,585 _RS26600 AZOBR_RS26605: AZOBR_RS26605 - TonB-denpendent receptor, at 112,582 to 115,071 _RS26605 AZOBR_RS26610: AZOBR_RS26610 - iron dicitrate transport regulator FecR, at 115,231 to 116,274 _RS26610
Group Condition AZOBR_RS26590 AZOBR_RS26595 AZOBR_RS26600 AZOBR_RS26605 AZOBR_RS26610
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.2 N.D. +0.1 -0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.1 -0.5 N.D. +0.1 -0.6
carbon source D,L-Lactate (C) -0.1 -0.2 N.D. -0.0 -0.6
stress 3-octanone 6.3476 mM -0.8 +0.3 N.D. +0.1 -0.4
nitrogen source L-Phenylalanine (N) -0.3 +0.1 N.D. +0.1 -0.6
stress Sisomicin 0.01 mg/ml -0.5 +0.2 N.D. -0.1 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.5 +0.1 N.D. +0.2 -0.3
stress 3-octanone 6.3476 mM +0.0 +0.1 N.D. +0.2 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 +0.1 N.D. +0.2 +0.0
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM +0.1 +0.1 N.D. +0.0 -0.6
stress 2-pentyl-furan 1.780666667 mM +0.2 +0.1 N.D. -0.0 -0.6
stress 3-octanone 6.3476 mM -0.2 +0.3 N.D. -0.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 -0.8 N.D. +0.0 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.5 +0.1 N.D. +0.2 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.2 N.D. -0.1 +0.7
carbon source D-Gluconic Acid (C) +0.1 -0.3 N.D. +0.0 +0.6
stress 3-octanone 5.2896 mM +0.7 +0.2 N.D. +0.0 -0.4
stress 2-pentyl-furan 1.335528 mM +1.0 +0.1 N.D. -0.2 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.0 N.D. -0.1 +0.8
stress 3-octanone 5.2896 mM +0.5 +0.0 N.D. +0.2 -0.2
motility outer cut, LB soft agar motility assay +0.0 -0.3 N.D. -0.0 +0.9
stress Cobalt chloride 0.2 mM +0.3 -0.1 N.D. -0.1 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.4 +0.3 N.D. +0.2 -0.1
stress 2-pentyl-furan 1.335528 mM +0.5 +0.1 N.D. -0.2 +0.3
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.4 +0.1 N.D. +0.2 +0.3
motility inner cut, LB soft agar motility assay +0.3 +0.2 N.D. +0.2 +0.4
stress Benzethonium chloride 0.003 mM +0.7 +0.1 N.D. +0.2 +0.3
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.3 +0.2 N.D. +0.1 +0.7
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.4 +0.3 N.D. +0.2 +0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.4 +0.3 N.D. +0.6 +0.4
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