Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS26400 and AZOBR_RS26405 are separated by 81 nucleotidesAZOBR_RS26405 and AZOBR_RS26410 overlap by 14 nucleotidesAZOBR_RS26410 and AZOBR_RS26415 are separated by 14 nucleotidesAZOBR_RS26415 and AZOBR_RS26420 are separated by 30 nucleotides AZOBR_RS26400: AZOBR_RS26400 - ABC transporter substrate-binding protein, at 68,834 to 69,637 _RS26400 AZOBR_RS26405: AZOBR_RS26405 - amino acid ABC transporter ATP-binding protein, at 69,719 to 70,447 _RS26405 AZOBR_RS26410: AZOBR_RS26410 - amino acid ABC transporter permease, at 70,434 to 71,084 _RS26410 AZOBR_RS26415: AZOBR_RS26415 - ABC transporter permease, at 71,099 to 71,758 _RS26415 AZOBR_RS26420: AZOBR_RS26420 - amino acid deaminase, at 71,789 to 73,087 _RS26420
Group Condition AZOBR_RS26400 AZOBR_RS26405 AZOBR_RS26410 AZOBR_RS26415 AZOBR_RS26420
stress 2-pentyl-furan 1.335528 mM -0.4 N.D. -0.3 -3.9 -0.1
carbon source D-Gluconic Acid (C) -0.3 N.D. -0.4 -0.5 -1.2
stress 2-pentyl-furan 1.780666667 mM -0.2 N.D. -0.4 -1.0 +0.4
stress Benzethonium chloride 0.003 mM -0.6 N.D. +0.0 +0.1 -0.7
stress 2-pentyl-furan 1.780666667 mM -0.4 N.D. -0.3 -0.7 +0.3
lb LB -0.2 N.D. -0.4 +0.3 -0.5
motility inner cut, LB soft agar motility assay -0.2 N.D. +0.1 -0.8 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.5 N.D. -0.5 -0.2 -0.5
stress Paraquat dichloride 0.02 mg/ml -0.1 N.D. +0.3 -0.5 -0.2
stress 3-octanone 6.3476 mM +0.0 N.D. -0.8 -0.1 +0.5
stress Chlorite 1 mM -0.3 N.D. +0.2 +0.4 -0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.2 N.D. +0.3 +0.2 -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.5 N.D. -0.3 +0.2 -0.6
stress 2-pentyl-furan 1.335528 mM +0.5 N.D. +0.2 -1.2 +0.3
nitrogen source L-Histidine (N) +0.3 N.D. -0.3 -0.6 +0.5
motility inner cut, LB soft agar motility assay +0.3 N.D. -0.2 +0.4 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 N.D. +0.0 -0.5 +0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.4 N.D. -0.3 -0.1 +0.6
anaerobic; rich media LB; anaerobic +0.2 N.D. +0.2 -0.2 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 N.D. +0.2 +0.3 +0.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.1 N.D. +0.1 +0.2 +0.8
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.1 N.D. +0.2 +0.4 +0.5
motility outer cut, LB soft agar motility assay +0.0 N.D. +0.6 +0.6 +0.2
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.5 N.D. +0.4 +0.6 +0.0
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.2 N.D. +0.3 +0.4 +0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.3 N.D. +0.6 +0.4 +0.5
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.4 N.D. +0.4 +0.4 +0.7
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.4 N.D. +0.6 +0.2 +0.7
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.4 N.D. +0.3 +0.4 +0.9
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.3 N.D. +0.7 +0.7 +1.1
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