Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS25660 and AZOBR_RS25665 are separated by 175 nucleotidesAZOBR_RS25665 and AZOBR_RS25670 are separated by 3 nucleotidesAZOBR_RS25670 and AZOBR_RS25675 are separated by 12 nucleotidesAZOBR_RS25675 and AZOBR_RS25680 are separated by 129 nucleotides AZOBR_RS25660: AZOBR_RS25660 - amidohydrolase, at 804,590 to 805,762 _RS25660 AZOBR_RS25665: AZOBR_RS25665 - hypothetical protein, at 805,938 to 806,531 _RS25665 AZOBR_RS25670: AZOBR_RS25670 - long-chain fatty acid--CoA ligase, at 806,535 to 809,945 _RS25670 AZOBR_RS25675: AZOBR_RS25675 - O-methyltransferase, at 809,958 to 810,551 _RS25675 AZOBR_RS25680: AZOBR_RS25680 - hypothetical protein, at 810,681 to 811,502 _RS25680
Group Condition AZOBR_RS25660 AZOBR_RS25665 AZOBR_RS25670 AZOBR_RS25675 AZOBR_RS25680
nitrogen source L-Isoleucine (N) -1.6 +0.1 -0.1 N.D. -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 -0.9 +0.0 N.D. -0.3
motility inner cut, LB soft agar motility assay +0.2 -1.0 -0.0 N.D. +0.1
stress 3-octanone 6.3476 mM -0.6 -0.1 +0.2 N.D. -0.2
stress Sisomicin 0.01 mg/ml -0.1 +0.3 -0.0 N.D. -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.6 +0.2 N.D. -0.4
stress Paraquat dichloride 0.02 mg/ml +0.4 -0.7 +0.1 N.D. -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -1.1 +0.0 N.D. +0.6
stress copper (II) chloride 2 mM -0.6 +0.3 +0.2 N.D. -0.0
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.3 -0.3 +0.6 N.D. +0.1
stress D-Cycloserine 0.125 mg/ml +0.9 -0.4 -0.0 N.D. -0.2
stress 3-octanone 5.2896 mM -0.4 +0.4 +0.1 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.4 +0.1 N.D. -0.4
anaerobic; rich media LB; anaerobic -0.1 -0.2 +0.5 N.D. +0.2
nitrogen source Cytosine (N) -0.4 +0.4 +0.3 N.D. +0.3
nitrogen source Glucuronamide (N) -0.3 +0.5 +0.2 N.D. +0.3
nitrogen source L-Threonine (N) -0.3 +0.4 -0.1 N.D. +0.7
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.4 +0.2 +0.6 N.D. +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.5 +0.2 +0.8 N.D. +0.3
carbon source D-Gluconic Acid (C) +0.3 +0.5 +0.4 N.D. -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.6 +0.6 +0.2 N.D. -0.2
carbon source butyrate (C) +0.1 +0.7 +0.0 N.D. +0.4
stress Benzethonium chloride 0.003 mM +0.6 +0.3 +0.2 N.D. +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.2 +0.4 +0.7 N.D. +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.0 +0.2 +0.7 N.D. +0.5
stress 2-pentyl-furan 2.671 mM +0.4 +0.4 +0.5 N.D. +0.0
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.2 +0.3 +0.7 N.D. +0.7
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.1 +0.6 +0.5 N.D. +0.3
motility inner cut, LB soft agar motility assay +0.9 +0.5 -0.3 N.D. +0.5
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.1 +0.5 +0.7 N.D. +0.5
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