Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS25650 and AZOBR_RS25655 are separated by 71 nucleotidesAZOBR_RS25655 and AZOBR_RS25660 are separated by 348 nucleotidesAZOBR_RS25660 and AZOBR_RS25665 are separated by 175 nucleotidesAZOBR_RS25665 and AZOBR_RS25670 are separated by 3 nucleotides AZOBR_RS25650: AZOBR_RS25650 - hypothetical protein, at 802,425 to 802,889 _RS25650 AZOBR_RS25655: AZOBR_RS25655 - oxidoreductase, at 802,961 to 804,241 _RS25655 AZOBR_RS25660: AZOBR_RS25660 - amidohydrolase, at 804,590 to 805,762 _RS25660 AZOBR_RS25665: AZOBR_RS25665 - hypothetical protein, at 805,938 to 806,531 _RS25665 AZOBR_RS25670: AZOBR_RS25670 - long-chain fatty acid--CoA ligase, at 806,535 to 809,945 _RS25670
Group Condition AZOBR_RS25650 AZOBR_RS25655 AZOBR_RS25660 AZOBR_RS25665 AZOBR_RS25670
nitrogen source L-Isoleucine (N) N.D. -0.3 -1.6 +0.1 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.0 -0.4 -0.9 +0.0
carbon source D-Gluconic Acid (C) N.D. -0.4 -0.2 -0.3 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.2 +0.3 -1.1 +0.0
stress Paraquat dichloride 0.04 mg/ml N.D. -0.3 -0.5 +0.3 -0.0
stress Paraquat dichloride 0.02 mg/ml N.D. -0.1 +0.4 -0.7 +0.1
motility inner cut, LB soft agar motility assay N.D. +0.5 +0.2 -1.0 -0.0
motility outer cut, LB soft agar motility assay N.D. +0.2 +0.2 -0.6 -0.1
stress copper (II) chloride 2 mM N.D. -0.2 -0.6 +0.3 +0.2
stress 2-pentyl-furan 1.780666667 mM N.D. +0.5 -0.0 -0.6 +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.1 +0.2 -0.6 +0.2
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days N.D. +0.2 -0.3 -0.3 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.6 +0.3 +0.4 +0.1
nitrogen source Cytosine (N) N.D. +0.1 -0.4 +0.4 +0.3
nitrogen source Glucuronamide (N) N.D. +0.2 -0.3 +0.5 +0.2
stress 3-octanone 5.2896 mM N.D. +0.3 -0.4 +0.4 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.3 -0.4 +0.2 +0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.2 -0.5 +0.2 +0.8
motility inner cut, LB soft agar motility assay N.D. -0.3 +0.9 +0.5 -0.3
motility inner cut, LB soft agar motility assay N.D. +0.4 +0.3 +0.3 -0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.3 -0.2 +0.3 +0.7
stress D-Cycloserine 0.125 mg/ml N.D. +0.5 +0.9 -0.4 -0.0
stress 2-pentyl-furan 2.671 mM N.D. -0.3 +0.4 +0.4 +0.5
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.3 -0.2 +0.4 +0.7
stress Benzethonium chloride 0.003 mM N.D. +0.2 +0.6 +0.3 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.5 -0.0 +0.2 +0.7
carbon source D-Gluconic Acid (C) N.D. +0.1 +0.3 +0.5 +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.3 +0.1 +0.5 +0.7
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.4 +0.1 +0.6 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.3 +0.6 +0.6 +0.2
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