Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS25635 and AZOBR_RS25645 are separated by 997 nucleotidesAZOBR_RS25645 and AZOBR_RS25650 are separated by 82 nucleotidesAZOBR_RS25650 and AZOBR_RS25655 are separated by 71 nucleotidesAZOBR_RS25655 and AZOBR_RS25660 are separated by 348 nucleotides AZOBR_RS25635: AZOBR_RS25635 - high-affinity branched-chain amino acid ABC transporter (permease protein) (fragment), at 799,246 to 800,232 _RS25635 AZOBR_RS25645: AZOBR_RS25645 - amino acid ABC transporter substrate-binding protein, at 801,230 to 802,342 _RS25645 AZOBR_RS25650: AZOBR_RS25650 - hypothetical protein, at 802,425 to 802,889 _RS25650 AZOBR_RS25655: AZOBR_RS25655 - oxidoreductase, at 802,961 to 804,241 _RS25655 AZOBR_RS25660: AZOBR_RS25660 - amidohydrolase, at 804,590 to 805,762 _RS25660
Group Condition AZOBR_RS25635 AZOBR_RS25645 AZOBR_RS25650 AZOBR_RS25655 AZOBR_RS25660
nitrogen source L-Isoleucine (N) -0.3 -0.2 N.D. -0.3 -1.6
stress Cobalt chloride 0.2 mM -0.4 -0.5 N.D. -0.3 -0.3
stress Paraquat dichloride 0.04 mg/ml -0.2 -0.3 N.D. -0.3 -0.5
stress copper (II) chloride 2 mM -0.2 -0.2 N.D. -0.2 -0.6
stress Cobalt chloride 0.3 mM -0.4 -0.5 N.D. -0.2 -0.1
nitrogen source L-Threonine (N) -0.3 -0.1 N.D. -0.1 -0.3
stress 3-octanone 6.3476 mM +0.0 -0.0 N.D. -0.1 -0.6
carbon source D-Gluconic Acid (C) -0.2 +0.2 N.D. -0.4 -0.2
stress 2-pentyl-furan 1.780666667 mM -0.1 +0.2 N.D. +0.1 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.1 N.D. -0.6 +0.3
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.3 -0.0 N.D. +0.2 -0.5
stress 2-pentyl-furan 1.780666667 mM +0.0 +0.2 N.D. +0.3 -0.5
stress 2-pentyl-furan 1.335528 mM +0.1 +0.3 N.D. +0.2 -0.4
lb LB +0.1 +0.1 N.D. +0.3 -0.4
stress 3-octanone 5.2896 mM +0.1 +0.1 N.D. +0.3 -0.4
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.2 +0.1 N.D. +0.3 -0.4
stress 2-pentyl-furan 2.671 mM +0.0 +0.2 N.D. -0.3 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.0 N.D. +0.1 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.3 N.D. -0.2 +0.3
stress 2-pentyl-furan 1.780666667 mM -0.1 +0.3 N.D. +0.5 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.1 +0.3 N.D. +0.2 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.3 +0.1 N.D. +0.5 -0.0
motility inner cut, LB soft agar motility assay +0.0 +0.0 N.D. +0.4 +0.3
stress Sisomicin 0.01 mg/ml +0.7 +0.1 N.D. +0.2 -0.1
motility inner cut, LB soft agar motility assay +0.1 +0.3 N.D. -0.3 +0.9
motility inner cut, LB soft agar motility assay +0.3 +0.1 N.D. +0.5 +0.2
stress Benzethonium chloride 0.003 mM +0.3 +0.0 N.D. +0.2 +0.6
motility outer cut, LB soft agar motility assay +0.3 +0.4 N.D. +0.2 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.3 +0.3 N.D. +0.3 +0.6
stress D-Cycloserine 0.125 mg/ml +0.4 +0.2 N.D. +0.5 +0.9
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