Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS25625 and AZOBR_RS25630 are separated by 23 nucleotidesAZOBR_RS25630 and AZOBR_RS25635 overlap by 4 nucleotidesAZOBR_RS25635 and AZOBR_RS25645 are separated by 997 nucleotidesAZOBR_RS25645 and AZOBR_RS25650 are separated by 82 nucleotides AZOBR_RS25625: AZOBR_RS25625 - leucine/isoleucine/valine transporter ATP-binding subunit, at 797,760 to 798,458 _RS25625 AZOBR_RS25630: AZOBR_RS25630 - ABC transporter, at 798,482 to 799,249 _RS25630 AZOBR_RS25635: AZOBR_RS25635 - high-affinity branched-chain amino acid ABC transporter (permease protein) (fragment), at 799,246 to 800,232 _RS25635 AZOBR_RS25645: AZOBR_RS25645 - amino acid ABC transporter substrate-binding protein, at 801,230 to 802,342 _RS25645 AZOBR_RS25650: AZOBR_RS25650 - hypothetical protein, at 802,425 to 802,889 _RS25650
Group Condition AZOBR_RS25625 AZOBR_RS25630 AZOBR_RS25635 AZOBR_RS25645 AZOBR_RS25650
nitrogen source L-Glutamic (N) N.D. -3.0 -0.1 +0.2 N.D.
stress Cobalt chloride 0.3 mM N.D. -1.0 -0.4 -0.5 N.D.
stress Cobalt chloride 0.2 mM N.D. -0.6 -0.4 -0.5 N.D.
stress 3-octanone 5.2896 mM N.D. -1.3 -0.1 +0.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.0 +0.1 -0.1 N.D.
nitrogen source L-Leucine (N) N.D. -1.0 +0.0 +0.0 N.D.
stress copper (II) chloride 2 mM N.D. -0.5 -0.2 -0.2 N.D.
stress 1-octen-3-ol 0.6030326667 mM N.D. -0.8 -0.2 +0.1 N.D.
stress Vancomycin 0.03 mg/ml N.D. -0.7 -0.2 +0.0 N.D.
carbon source D-Gluconic Acid (C) N.D. -0.8 -0.2 +0.2 N.D.
stress 1-octen-3-ol 0.6030326667 mM N.D. -0.7 -0.0 +0.2 N.D.
stress Benzethonium chloride 0.003 mM N.D. -0.7 +0.3 +0.0 N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. -0.6 +0.2 +0.1 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. -0.6 +0.3 -0.0 N.D.
stress Paraquat dichloride 0.04 mg/ml N.D. +0.4 -0.2 -0.3 N.D.
nitrogen source L-Isoleucine (N) N.D. +0.6 -0.3 -0.2 N.D.
carbon source butyrate (C) N.D. +0.8 -0.1 -0.1 N.D.
nitrogen source L-Aspartic Acid (N) N.D. +0.6 -0.2 +0.2 N.D.
carbon source pyruvate (C) N.D. +0.8 +0.1 -0.1 N.D.
stress D-Cycloserine 0.125 mg/ml N.D. +0.3 +0.4 +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.8 +0.2 -0.1 N.D.
nitrogen source Urea (N) N.D. +1.0 -0.1 +0.0 N.D.
nitrogen source Glucuronamide (N) N.D. +0.8 +0.1 +0.1 N.D.
lb LB N.D. +0.7 +0.3 -0.0 N.D.
stress Sisomicin 0.01 mg/ml N.D. +0.3 +0.7 +0.1 N.D.
nitrogen source L-Phenylalanine (N) N.D. +1.2 -0.0 -0.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.7 +0.1 +0.3 N.D.
motility outer cut, LB soft agar motility assay N.D. +0.4 +0.3 +0.4 N.D.
nitrogen source L-Citrulline (N) N.D. +1.3 -0.0 -0.0 N.D.
motility inner cut, LB soft agar motility assay N.D. +1.0 +0.1 +0.3 N.D.
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