Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS25620 and AZOBR_RS25625 are separated by 63 nucleotidesAZOBR_RS25625 and AZOBR_RS25630 are separated by 23 nucleotidesAZOBR_RS25630 and AZOBR_RS25635 overlap by 4 nucleotidesAZOBR_RS25635 and AZOBR_RS25645 are separated by 997 nucleotides AZOBR_RS25620: AZOBR_RS25620 - 2-amino-thiazoline-4-carboxylic acid hydrolase, at 797,232 to 797,696 _RS25620 AZOBR_RS25625: AZOBR_RS25625 - leucine/isoleucine/valine transporter ATP-binding subunit, at 797,760 to 798,458 _RS25625 AZOBR_RS25630: AZOBR_RS25630 - ABC transporter, at 798,482 to 799,249 _RS25630 AZOBR_RS25635: AZOBR_RS25635 - high-affinity branched-chain amino acid ABC transporter (permease protein) (fragment), at 799,246 to 800,232 _RS25635 AZOBR_RS25645: AZOBR_RS25645 - amino acid ABC transporter substrate-binding protein, at 801,230 to 802,342 _RS25645
Group Condition AZOBR_RS25620 AZOBR_RS25625 AZOBR_RS25630 AZOBR_RS25635 AZOBR_RS25645
nitrogen source L-Glutamic (N) -0.2 N.D. -3.0 -0.1 +0.2
stress Cobalt chloride 0.3 mM -0.7 N.D. -1.0 -0.4 -0.5
stress Cobalt chloride 0.2 mM -0.2 N.D. -0.6 -0.4 -0.5
nitrogen source L-Leucine (N) -0.7 N.D. -1.0 +0.0 +0.0
stress 3-octanone 6.3476 mM -1.7 N.D. +0.1 +0.0 -0.0
nitrogen source L-Phenylalanine (N) -1.1 N.D. -0.5 -0.0 +0.2
stress 1-octen-3-ol 0.6030326667 mM -0.5 N.D. -0.8 -0.2 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 N.D. -1.0 +0.1 -0.1
carbon source D-Gluconic Acid (C) -0.4 N.D. -0.8 -0.2 +0.2
stress Vancomycin 0.03 mg/ml -0.3 N.D. -0.7 -0.2 +0.0
stress 3-octanone 5.2896 mM +0.1 N.D. -1.3 -0.1 +0.2
stress 2-pentyl-furan 1.335528 mM -0.8 N.D. +0.4 -0.3 +0.1
stress 3-octanone 6.3476 mM -1.0 N.D. +0.3 -0.0 +0.1
nitrogen source nitrate (N) -1.3 N.D. +0.5 +0.0 +0.2
nitrogen source Urea (N) -0.9 N.D. +0.5 -0.0 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.4 N.D. -0.6 +0.3 -0.0
carbon source D-Fructose (C) -0.4 N.D. +0.6 -0.1 +0.1
nitrogen source Glucuronamide (N) -0.7 N.D. +0.8 +0.1 +0.1
lb LB -0.6 N.D. +0.7 +0.3 -0.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.7 N.D. -0.5 +0.3 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.9 N.D. -0.4 +0.2 -0.0
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.9 N.D. -0.4 +0.2 +0.0
nitrogen source L-Isoleucine (N) +0.8 N.D. +0.6 -0.3 -0.2
carbon source butyrate (C) +0.4 N.D. +0.8 -0.1 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 N.D. +0.6 -0.1 -0.1
nitrogen source L-Citrulline (N) +0.1 N.D. +1.3 -0.0 -0.0
lb LB +0.7 N.D. +0.6 +0.3 +0.0
motility outer cut, LB soft agar motility assay +0.6 N.D. +0.4 +0.3 +0.4
nitrogen source L-Phenylalanine (N) +0.9 N.D. +1.2 -0.0 -0.2
motility inner cut, LB soft agar motility assay +0.7 N.D. +1.0 +0.1 +0.3
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AZOBR_RS25620
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AZOBR_RS25645
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