Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS24530 and AZOBR_RS24535 overlap by 7 nucleotidesAZOBR_RS24535 and AZOBR_RS24540 are separated by 13 nucleotidesAZOBR_RS24540 and AZOBR_RS24545 overlap by 4 nucleotidesAZOBR_RS24545 and AZOBR_RS24550 are separated by 13 nucleotides AZOBR_RS24530: AZOBR_RS24530 - formylmethanofuran dehydrogenase, at 561,236 to 562,507 _RS24530 AZOBR_RS24535: AZOBR_RS24535 - formylmethanofuran dehydrogenase subunit A, at 562,501 to 564,162 _RS24535 AZOBR_RS24540: AZOBR_RS24540 - formylmethanofuran--tetrahydromethanopterin formyltransferase, at 564,176 to 565,075 _RS24540 AZOBR_RS24545: AZOBR_RS24545 - putative formylmethanofuran dehydrogenase subunit C, at 565,072 to 565,896 _RS24545 AZOBR_RS24550: AZOBR_RS24550 - branched-chain amino acid ABC transporter substrate-binding protein, at 565,910 to 567,100 _RS24550
Group Condition AZOBR_RS24530 AZOBR_RS24535 AZOBR_RS24540 AZOBR_RS24545 AZOBR_RS24550
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.5 -0.6 N.D. -0.8
stress 2-pentyl-furan 1.780666667 mM N.D. -0.8 -0.7 N.D. -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.5 -0.1 N.D. -0.7
stress 2-pentyl-furan 1.335528 mM N.D. -0.7 -0.6 N.D. +0.0
stress 2-pentyl-furan 2.671 mM N.D. -0.9 -0.7 N.D. +0.5
stress 2-pentyl-furan 1.780666667 mM N.D. -0.3 -0.3 N.D. -0.4
nitrogen source L-Glutamic (N) N.D. -0.5 -0.5 N.D. -0.1
stress Paraquat dichloride 0.04 mg/ml N.D. -0.8 -0.3 N.D. +0.1
motility outer cut, LB soft agar motility assay N.D. -0.5 -0.4 N.D. +0.0
carbon source Glycerol (C) N.D. +0.0 -0.4 N.D. -0.4
stress 3-octanone 6.3476 mM N.D. -0.9 +0.1 N.D. +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.5 +0.2 N.D. -0.4
carbon source D-Gluconic Acid (C) N.D. +0.1 -0.4 N.D. -0.4
lb no stress control LB no stress control N.D. -0.6 -0.1 N.D. +0.2
motility inner cut, LB soft agar motility assay N.D. -0.1 -0.7 N.D. +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.1 +0.2 N.D. -0.6
stress 3-octanone 5.2896 mM N.D. +0.4 -0.4 N.D. -0.2
stress Benzethonium chloride 0.003 mM N.D. -0.4 -0.1 N.D. +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.4 -0.3 N.D. -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.2 +0.2 N.D. -0.5
stress 2-pentyl-furan 1.335528 mM N.D. -0.5 +0.6 N.D. +0.1
nitrogen source casaminos (N) N.D. -0.3 +0.4 N.D. +0.4
nitrogen source L-Aspartic Acid (N) N.D. +0.4 -0.1 N.D. +0.4
nitrogen source L-Phenylalanine (N) N.D. +0.1 +0.4 N.D. +0.4
stress Sisomicin 0.01 mg/ml N.D. -0.3 +0.5 N.D. +0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.3 +0.3 N.D. +0.4
carbon source butyrate (C) N.D. +0.3 +0.3 N.D. +0.5
nitrogen source L-Histidine (N) N.D. +0.4 +0.5 N.D. +0.2
stress 2-pentyl-furan 1.335528 mM N.D. +0.4 +0.4 N.D. +0.3
nitrogen source L-Isoleucine (N) N.D. +0.7 +0.4 N.D. +0.5
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