Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS24290 and AZOBR_RS24295 are separated by 53 nucleotidesAZOBR_RS24295 and AZOBR_RS24300 are separated by 201 nucleotidesAZOBR_RS24300 and AZOBR_RS24310 are separated by 2646 nucleotidesAZOBR_RS24310 and AZOBR_RS24315 are separated by 31 nucleotides AZOBR_RS24290: AZOBR_RS24290 - ribonucleotide-diphosphate reductase subunit alpha, at 495,877 to 497,751 _RS24290 AZOBR_RS24295: AZOBR_RS24295 - ribonucleotide-diphosphate reductase subunit beta, at 497,805 to 498,896 _RS24295 AZOBR_RS24300: AZOBR_RS24300 - methylcitrate synthase, at 499,098 to 500,261 _RS24300 AZOBR_RS24310: AZOBR_RS24310 - 3-methylitaconate isomerase, at 502,908 to 504,113 _RS24310 AZOBR_RS24315: AZOBR_RS24315 - carboxyvinyl-carboxyphosphonate phosphorylmutase, at 504,145 to 505,005 _RS24315
Group Condition AZOBR_RS24290 AZOBR_RS24295 AZOBR_RS24300 AZOBR_RS24310 AZOBR_RS24315
nitrogen source L-Histidine (N) -0.6 -0.6 -0.0 -0.8 -0.3
stress D-Cycloserine 0.125 mg/ml -0.4 -0.5 -0.2 -0.3 +0.3
stress Cobalt chloride 0.2 mM -0.2 -0.0 -0.1 -0.4 -0.5
motility inner cut, LB soft agar motility assay +0.1 -0.1 +0.1 -0.8 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.2 +0.1 -0.4 -0.4
motility inner cut, LB soft agar motility assay -0.1 -0.2 -0.1 -0.7 +0.1
carbon source D-Gluconic Acid (C) +0.1 -0.0 -0.2 -0.5 -0.2
nitrogen source L-Lysine (N) -0.1 +0.0 +0.0 -0.8 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.1 -0.2 -0.3 +0.1 -0.4
motility outer cut, LB soft agar motility assay -0.5 +0.0 -0.2 +0.2 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 -0.4 -0.3 -0.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 +0.2 -0.5 -0.4 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 -0.1 -0.2 -0.5 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.4 -0.4 -0.4 +0.7
stress 1-octen-3-ol 0.6784116667 mM -0.5 -0.2 +0.0 +0.1 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.1 -0.1 -0.5 +0.6
stress Benzethonium chloride 0.003 mM +0.1 -0.1 +0.1 -0.6 +0.1
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.1 +0.4 +0.0 +0.0 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.2 -0.2 -0.4 +1.1
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM +0.2 +0.1 +0.1 +0.4 -0.2
lb no stress control LB no stress control -0.1 +0.2 -0.1 +0.4 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.1 +0.0 +0.2 +0.7 -0.2
stress 2-pentyl-furan 2.671 mM -0.0 +0.4 -0.3 +0.3 +0.3
motility inner cut, LB soft agar motility assay +0.4 -0.2 +0.2 +0.8 -0.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.2 +0.3 +0.2 +0.3 -0.2
stress 2-pentyl-furan 1.780666667 mM +0.6 +0.0 +0.1 -0.3 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.2 +0.1 +0.0 +0.6 -0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.2 +0.3 +0.2 +0.3 -0.1
stress 2-pentyl-furan 1.335528 mM +0.1 +0.2 -0.1 +0.4 +0.3
stress 2-pentyl-furan 1.780666667 mM +0.1 +0.2 -0.2 +0.5 +0.3
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