Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS23680 and AZOBR_RS23685 are separated by 787 nucleotidesAZOBR_RS23685 and AZOBR_RS23695 are separated by 1147 nucleotidesAZOBR_RS23695 and AZOBR_RS23700 are separated by 47 nucleotidesAZOBR_RS23700 and AZOBR_RS23705 are separated by 203 nucleotides AZOBR_RS23680: AZOBR_RS23680 - hypothetical protein, at 346,621 to 347,577 _RS23680 AZOBR_RS23685: AZOBR_RS23685 - AsnC family transcriptional regulator, at 348,365 to 348,826 _RS23685 AZOBR_RS23695: AZOBR_RS23695 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data), at 349,974 to 353,681 _RS23695 AZOBR_RS23700: AZOBR_RS23700 - hypothetical protein, at 353,729 to 353,917 _RS23700 AZOBR_RS23705: AZOBR_RS23705 - beta-lactamase, at 354,121 to 355,170 _RS23705
Group Condition AZOBR_RS23680 AZOBR_RS23685 AZOBR_RS23695 AZOBR_RS23700 AZOBR_RS23705
carbon source L-Proline (C) +0.0 -4.0 -4.5 N.D. -0.1
carbon source L-Proline (C) +0.0 -3.9 -4.3 N.D. -0.1
nitrogen source L-Citrulline (N) -0.3 -0.4 -1.4 N.D. +0.1
stress 2-pentyl-furan 1.780666667 mM -0.9 -0.7 -0.2 N.D. +0.2
stress 2-pentyl-furan 1.335528 mM -0.6 -1.0 +0.0 N.D. -0.0
nitrogen source L-Threonine (N) -0.7 -0.6 -0.1 N.D. -0.0
carbon source D-Gluconic Acid (C) +0.2 -1.1 -0.2 N.D. +0.1
stress 3-octanone 5.2896 mM -0.5 -0.5 +0.0 N.D. +0.1
nitrogen source L-Glutamic (N) -0.5 -0.1 -0.3 N.D. +0.0
stress D-Cycloserine 0.125 mg/ml +0.1 -0.7 -0.3 N.D. +0.1
stress 3-octanone 6.3476 mM -0.5 -0.2 -0.2 N.D. +0.1
nitrogen source L-Lysine (N) -0.1 -0.5 -0.4 N.D. +0.2
motility inner cut, LB soft agar motility assay +0.6 -1.2 +0.1 N.D. -0.1
stress Cobalt chloride 0.3 mM +0.6 -0.7 -0.2 N.D. -0.1
lb LB +0.6 -0.2 -0.2 N.D. -0.5
lb LB +0.3 -0.4 -0.3 N.D. +0.2
motility inner cut, LB soft agar motility assay -0.7 +0.4 +0.1 N.D. +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.5 +0.5 +0.1 N.D. -0.0
nitrogen source L-Histidine (N) +0.6 -0.4 +0.0 N.D. +0.2
anaerobic; rich media LB; anaerobic +0.5 -0.2 +0.3 N.D. -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.4 -0.3 +0.4 N.D. +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.7 +0.1 N.D. -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 +0.7 +0.1 N.D. -0.2
stress 2-pentyl-furan 1.780666667 mM +0.7 +0.2 +0.2 N.D. -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.7 +0.0 +0.1 N.D. -0.0
motility inner cut, LB soft agar motility assay +0.3 -0.0 +0.4 N.D. +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.5 +0.3 +0.4 N.D. -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.3 +0.8 -0.1 N.D. -0.0
stress 2-pentyl-furan 1.335528 mM +0.9 -0.2 +0.3 N.D. +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.6 +0.2 +0.4 N.D. +0.1
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