Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS23305 and AZOBR_RS23310 are separated by 35 nucleotidesAZOBR_RS23310 and AZOBR_RS23315 are separated by 53 nucleotidesAZOBR_RS23315 and AZOBR_RS23320 overlap by 7 nucleotidesAZOBR_RS23320 and AZOBR_RS23325 are separated by 466 nucleotides AZOBR_RS23305: AZOBR_RS23305 - hypothetical protein, at 254,386 to 255,366 _RS23305 AZOBR_RS23310: AZOBR_RS23310 - hypothetical protein, at 255,402 to 256,379 _RS23310 AZOBR_RS23315: AZOBR_RS23315 - conserved membrane protein of unknown function, at 256,433 to 256,918 _RS23315 AZOBR_RS23320: AZOBR_RS23320 - C4-dicarboxylate ABC transporter permease, at 256,912 to 258,414 _RS23320 AZOBR_RS23325: AZOBR_RS23325 - RNA polymerase sigma70, at 258,881 to 259,414 _RS23325
Group Condition AZOBR_RS23305 AZOBR_RS23310 AZOBR_RS23315 AZOBR_RS23320 AZOBR_RS23325
nitrogen source L-Histidine (N) -0.1 -3.3 -0.5 -0.0 +0.2
nitrogen source L-Citrulline (N) -0.2 -2.0 +0.1 -0.2 +0.1
carbon source L-Glutamine (C) -0.0 -1.5 -0.3 +0.1 -0.2
nitrogen source Cytosine (N) -0.2 -1.4 -0.1 -0.1 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -1.3 +0.1 +0.1 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -1.8 -0.1 +0.4 -0.1
nitrogen source Urea (N) +0.0 -1.6 +0.0 +0.0 +0.2
stress 3-octanone 6.3476 mM -0.5 -0.5 -0.5 -0.1 +0.3
carbon source a-Ketoglutaric (C) +0.2 -1.4 +0.0 -0.2 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 -0.9 +0.1 -0.4 +0.8
nitrogen source Parabanic Acid (N) -0.1 -1.2 +0.1 -0.1 +0.3
nitrogen source Parabanic Acid (N) +0.0 -1.1 -0.1 -0.1 +0.3
carbon source D-Gluconic Acid (C) +0.1 -1.5 +0.1 +0.1 +0.2
nitrogen source L-Leucine (N) -0.0 -1.1 +0.1 -0.1 +0.4
stress 3-octanone 5.2896 mM +0.2 -0.8 -0.4 -0.1 +0.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.3 -0.7 +0.2 -0.3 +0.4
stress 3-octanone 6.3476 mM +0.3 +0.3 -0.3 -0.1 -0.7
motility inner cut, LB soft agar motility assay +0.1 +0.7 -0.8 -0.3 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.3 -0.1 +0.4 -0.4 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -0.6 +0.3 +0.3 +0.7
motility outer cut, LB soft agar motility assay +0.3 +1.2 -0.0 -0.5 -0.5
stress Aluminum chloride 5 mM -0.0 +1.2 -0.1 -0.1 -0.4
stress Paraquat dichloride 0.02 mg/ml -0.2 +1.2 -0.6 -0.0 +0.3
stress benzoic 0.145 mg/ml +0.1 +1.1 -0.3 +0.0 -0.1
lb LB -0.2 +1.1 -0.1 -0.1 +0.2
nitrogen source L-Isoleucine (N) +0.1 -0.3 +0.8 +0.1 +0.5
stress Paraquat dichloride 0.04 mg/ml +0.1 +1.4 -0.5 -0.1 +0.4
stress copper (II) chloride 2 mM +0.2 +0.8 +0.2 -0.1 +0.4
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.0 +1.2 +0.2 -0.0 +0.5
motility inner cut, LB soft agar motility assay +0.5 -0.3 +0.7 +0.3 +0.8
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