Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS23065 and AZOBR_RS23070 are separated by 134 nucleotidesAZOBR_RS23070 and AZOBR_RS23080 are separated by 2535 nucleotidesAZOBR_RS23080 and AZOBR_RS23085 overlap by 4 nucleotidesAZOBR_RS23085 and AZOBR_RS23090 are separated by 192 nucleotides AZOBR_RS23065: AZOBR_RS23065 - LacI family transcription regulator, at 189,761 to 190,846 _RS23065 AZOBR_RS23070: AZOBR_RS23070 - ABC transporter substrate-binding protein, at 190,981 to 192,036 _RS23070 AZOBR_RS23080: AZOBR_RS23080 - ribulose 1 5-bisphosphate carboxylase, at 194,572 to 195,843 _RS23080 AZOBR_RS23085: AZOBR_RS23085 - type III effector Hrp-dependent outer protein, at 195,840 to 197,147 _RS23085 AZOBR_RS23090: AZOBR_RS23090 - Crp/Fnr family transcription regulator, at 197,340 to 198,071 _RS23090
Group Condition AZOBR_RS23065 AZOBR_RS23070 AZOBR_RS23080 AZOBR_RS23085 AZOBR_RS23090
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.2 +0.0 N.D. +0.2 -4.4
stress Chlorite 1 mM -0.0 -0.3 N.D. -0.8 -2.8
nitrogen source L-Histidine (N) +0.3 +0.3 N.D. +0.0 -4.1
stress Paraquat dichloride 0.02 mg/ml +0.2 -0.1 N.D. +0.0 -3.3
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.2 -0.1 N.D. -0.2 -2.6
motility outer cut, LB soft agar motility assay -0.3 -0.1 N.D. +0.2 -2.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.2 +0.5 N.D. +0.0 -2.8
carbon source D-Gluconic Acid (C) -0.3 -0.3 N.D. +0.1 -1.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.4 N.D. -0.4 -2.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 +0.2 N.D. +0.1 -2.3
anaerobic; rich media LB; anaerobic -0.3 +0.1 N.D. +0.1 -2.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.3 -0.3 N.D. -0.1 -2.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -0.1 N.D. +0.0 -2.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 +0.1 N.D. +0.3 -2.3
nitrogen source L-Isoleucine (N) +1.0 -0.1 N.D. -0.5 -2.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -0.0 N.D. +0.1 -2.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.1 +0.1 N.D. -0.2 -1.9
stress benzoic 0.145 mg/ml -0.2 +0.2 N.D. -0.1 -1.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.2 N.D. +0.4 -2.2
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days +0.3 +0.2 N.D. -0.0 -1.9
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.2 +0.1 N.D. +0.3 -2.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.5 +0.3 N.D. +0.2 -2.4
stress 3-octanone 6.3476 mM +0.1 -0.9 N.D. +0.4 -0.8
nitrogen source Urea (N) +0.1 +0.3 N.D. +0.2 -1.8
nitrogen source Parabanic Acid (N) +0.4 -0.0 N.D. +0.1 -1.6
stress 2-pentyl-furan 1.780666667 mM +0.4 +0.5 N.D. -0.1 -1.8
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.1 +0.3 N.D. +0.5 -1.5
carbon source Glycerol (C) +0.3 +0.5 N.D. +0.5 -1.0
stress 2-pentyl-furan 2.671 mM -0.1 +0.3 N.D. -0.5 +1.4
motility inner cut, LB soft agar motility assay -0.2 -0.7 N.D. +0.4 +2.0
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