Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS22740 and AZOBR_RS22745 are separated by 280 nucleotidesAZOBR_RS22745 and AZOBR_RS22750 are separated by 51 nucleotidesAZOBR_RS22750 and AZOBR_RS22755 are separated by 46 nucleotidesAZOBR_RS22755 and AZOBR_RS22760 are separated by 87 nucleotides AZOBR_RS22740: AZOBR_RS22740 - transcriptional regulator, at 110,032 to 110,871 _RS22740 AZOBR_RS22745: AZOBR_RS22745 - ATPase AAA, at 111,152 to 113,818 _RS22745 AZOBR_RS22750: AZOBR_RS22750 - glutamyl-tRNA(Gln) amidotransferase, at 113,870 to 115,282 _RS22750 AZOBR_RS22755: AZOBR_RS22755 - dihydropyrimidinase, at 115,329 to 116,765 _RS22755 AZOBR_RS22760: AZOBR_RS22760 - Asp/Glu/hydantoin racemase, at 116,853 to 117,584 _RS22760
Group Condition AZOBR_RS22740 AZOBR_RS22745 AZOBR_RS22750 AZOBR_RS22755 AZOBR_RS22760
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -0.1 -0.5 -0.5 -0.2
carbon source Glycerol (C) -0.7 +0.1 -0.1 -0.1 -0.5
nitrogen source casaminos (N) -0.3 -0.0 -0.0 +0.0 -0.9
carbon source D-Gluconic Acid (C) -0.1 -0.1 -0.2 -0.2 -0.6
stress 2-pentyl-furan 1.780666667 mM -0.3 -0.1 -0.1 -0.3 -0.5
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.2 +0.0 -0.1 -0.2 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 +0.0 -0.1 -0.2 -0.2
carbon source L-Proline (C) -0.4 +0.1 -0.2 -0.1 -0.4
carbon source Glycerol (C) -0.6 -0.1 -0.1 -0.3 -0.0
motility inner cut, LB soft agar motility assay -0.2 -0.5 -0.2 -0.1 +0.2
nitrogen source L-Threonine (N) +0.0 -0.1 +0.1 -0.0 -0.8
nitrogen source nitrate (N) -0.2 +0.2 +0.0 -0.3 -0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.0 +0.1 +0.2 -0.3 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 +0.3 -0.5 +0.0 -0.3
stress 2-pentyl-furan 1.335528 mM -0.5 -0.3 -0.0 +0.1 +0.2
stress 3-octanone 6.3476 mM -0.4 -0.3 -0.1 -0.1 +0.4
nitrogen source L-Glutamic (N) -0.5 +0.2 -0.1 -0.0 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 +0.4 +0.1 -0.2 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.3 +0.1 +0.0 +0.4 -0.4
stress 2-pentyl-furan 1.780666667 mM -0.6 +0.2 -0.0 +0.2 +0.4
stress Cobalt chloride 0.2 mM -0.1 -0.1 -0.4 +0.2 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 +0.1 +0.2 -0.0 +0.7
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.1 +0.4 +0.3 -0.1 -0.1
stress Paraquat dichloride 0.02 mg/ml +0.5 +0.1 -0.3 +0.2 +0.3
stress 2-pentyl-furan 2.671 mM +0.1 -0.0 +0.1 +0.2 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.2 +0.1 +0.2 +0.6
lb LB +0.1 +0.1 -0.1 +0.3 +0.7
nitrogen source L-Isoleucine (N) +0.0 +0.0 +0.2 +0.2 +0.8
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.4 +0.2 +0.5 +0.2 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.1 +0.2 +0.4 +0.1 +0.5
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