Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS22495 and AZOBR_RS22500 are separated by 293 nucleotidesAZOBR_RS22500 and AZOBR_RS22505 are separated by 14 nucleotidesAZOBR_RS22505 and AZOBR_RS22510 are separated by 176 nucleotidesAZOBR_RS22510 and AZOBR_RS22515 are separated by 79 nucleotides AZOBR_RS22495: AZOBR_RS22495 - GntR family transcriptional regulator, at 57,746 to 58,432 _RS22495 AZOBR_RS22500: AZOBR_RS22500 - methylmalonate-semialdehyde dehydrogenase, at 58,726 to 60,225 _RS22500 AZOBR_RS22505: AZOBR_RS22505 - omega amino acid--pyruvate aminotransferase, at 60,240 to 61,550 _RS22505 AZOBR_RS22510: AZOBR_RS22510 - LysR family transcriptional regulator, at 61,727 to 62,689 _RS22510 AZOBR_RS22515: AZOBR_RS22515 - membrane protein of unknown function, at 62,769 to 63,329 _RS22515
Group Condition AZOBR_RS22495 AZOBR_RS22500 AZOBR_RS22505 AZOBR_RS22510 AZOBR_RS22515
stress 2-pentyl-furan 1.780666667 mM -0.4 -0.3 +0.1 -0.9 N.D.
motility outer cut, LB soft agar motility assay -0.1 -1.2 -0.1 +0.1 N.D.
stress 2-pentyl-furan 1.335528 mM -0.5 -0.4 -0.2 +0.1 N.D.
lb LB -0.1 -0.6 -0.3 +0.0 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.1 -0.0 -0.1 -0.8 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -0.6 +0.1 -0.5 N.D.
nitrogen source L-Glutamic (N) +0.3 -0.7 -0.0 -0.3 N.D.
nitrogen source L-Leucine (N) +0.1 -0.8 +0.1 -0.2 N.D.
stress Benzethonium chloride 0.003 mM -0.2 +0.3 +0.1 -1.0 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.2 -0.4 -0.2 +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.8 +0.2 +0.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.4 +0.2 +0.1 N.D.
nitrogen source L-Threonine (N) -0.2 -0.4 -0.2 +0.3 N.D.
carbon source L-Lactate (C) +0.1 +0.0 +0.1 -0.6 N.D.
nitrogen source Ammonium chloride (N) -0.1 +0.4 +0.0 -0.6 N.D.
stress 3-octanone 6.3476 mM -0.2 -0.2 +0.4 -0.3 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.1 -0.3 -0.1 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -0.9 +0.2 +0.2 N.D.
nitrogen source L-Phenylalanine (N) -0.3 +0.5 +0.0 -0.2 N.D.
stress Cobalt chloride 0.2 mM +0.2 -0.5 -0.1 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.5 -0.1 +0.5 N.D.
stress 2-pentyl-furan 2.671 mM -0.4 +0.1 -0.0 +0.4 N.D.
stress Cobalt chloride 0.3 mM +0.2 -0.2 +0.2 +0.2 N.D.
stress 2-pentyl-furan 1.780666667 mM -0.2 +0.3 +0.5 -0.0 N.D.
stress 2-pentyl-furan 1.335528 mM +0.4 +0.1 -0.3 +0.4 N.D.
motility inner cut, LB soft agar motility assay +0.0 +0.4 +0.0 +0.4 N.D.
nitrogen source L-Isoleucine (N) +0.5 +0.4 +0.2 +0.0 N.D.
stress D-Cycloserine 0.125 mg/ml +0.5 +0.7 -0.1 +0.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.1 +0.1 +0.8 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.2 +0.4 +1.2 N.D.
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