Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS21725 and AZOBR_RS21730 are separated by 45 nucleotidesAZOBR_RS21730 and AZOBR_RS21735 overlap by 4 nucleotidesAZOBR_RS21735 and AZOBR_RS21740 are separated by 43 nucleotidesAZOBR_RS21740 and AZOBR_RS21750 are separated by 1872 nucleotides AZOBR_RS21725: AZOBR_RS21725 - imidazolonepropionase, at 1,650,274 to 1,651,488 _RS21725 AZOBR_RS21730: AZOBR_RS21730 - hypothetical protein, at 1,651,534 to 1,651,782 _RS21730 AZOBR_RS21735: AZOBR_RS21735 - hypothetical protein, at 1,651,779 to 1,652,204 _RS21735 AZOBR_RS21740: AZOBR_RS21740 - urocanate hydratase, at 1,652,248 to 1,653,936 _RS21740 AZOBR_RS21750: AZOBR_RS21750 - S-adenosyl-L-methionine (SAM)-dependent methyltransferase PhcB, at 1,655,809 to 1,656,573 _RS21750
Group Condition AZOBR_RS21725 AZOBR_RS21730 AZOBR_RS21735 AZOBR_RS21740 AZOBR_RS21750
motility outer cut, LB soft agar motility assay -0.3 N.D. -0.2 -0.3 -0.9
stress 2-pentyl-furan 1.780666667 mM -0.0 N.D. -0.5 +0.1 -1.2
nitrogen source L-Serine (N) -0.1 N.D. -0.2 -0.0 -1.3
stress Cobalt chloride 0.3 mM +0.1 N.D. -0.0 -0.2 -1.4
nitrogen source L-Threonine (N) -0.1 N.D. -0.3 -0.3 -0.8
nitrogen source L-Phenylalanine (N) +0.0 N.D. -0.3 -0.2 -1.0
stress 3-octanone 6.3476 mM -0.5 N.D. +0.3 -0.6 -0.5
nitrogen source L-Histidine (N) -0.2 N.D. -0.7 -0.5 -0.0
nitrogen source Parabanic Acid (N) +0.2 N.D. -0.2 +0.0 -1.3
nitrogen source L-Isoleucine (N) -0.5 N.D. -0.1 +0.0 -0.7
nitrogen source Cytosine (N) +0.0 N.D. -0.0 +0.1 -1.3
nitrogen source D-Alanine (N) +0.1 N.D. -0.2 -0.1 -0.9
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.2 N.D. +0.3 +0.1 -0.9
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.0 N.D. +0.1 +0.2 -1.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.1 N.D. +0.3 +0.2 -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 N.D. +0.2 -0.3 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 N.D. +0.8 -0.1 +0.2
nitrogen source L-Glutamic (N) -0.2 N.D. +0.7 -0.2 +0.4
stress 1-octen-3-ol 0.6030326667 mM -0.3 N.D. +0.1 +0.0 +1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.6 N.D. +0.3 +0.1 +1.1
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM -0.1 N.D. +0.1 +0.1 +1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.2 N.D. +0.6 +0.2 +0.5
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM -0.1 N.D. +0.1 +0.1 +1.1
stress 3-octanone 6.3476 mM +0.2 N.D. +0.2 -0.1 +1.0
stress 2-pentyl-furan 1.335528 mM +0.4 N.D. +0.3 -0.2 +1.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.3 N.D. +0.5 +0.2 +0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.1 N.D. +0.9 +0.1 +0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 N.D. +2.4 +0.3 -1.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.2 N.D. +0.6 +0.1 +1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 N.D. +0.8 +0.2 +1.6
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