Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS21555 and AZOBR_RS21565 are separated by 2204 nucleotidesAZOBR_RS21565 and AZOBR_RS21570 are separated by 90 nucleotidesAZOBR_RS21570 and AZOBR_RS21575 overlap by 4 nucleotidesAZOBR_RS21575 and AZOBR_RS21580 are separated by 15 nucleotides AZOBR_RS21555: AZOBR_RS21555 - nucleotidyl transferase, at 1,611,726 to 1,612,451 _RS21555 AZOBR_RS21565: AZOBR_RS21565 - hypothetical protein, at 1,614,656 to 1,616,038 _RS21565 AZOBR_RS21570: AZOBR_RS21570 - N-acetylneuraminate synthase, at 1,616,129 to 1,617,181 _RS21570 AZOBR_RS21575: AZOBR_RS21575 - spore coat protein, at 1,617,178 to 1,617,936 _RS21575 AZOBR_RS21580: AZOBR_RS21580 - hypothetical protein, at 1,617,952 to 1,618,185 _RS21580
Group Condition AZOBR_RS21555 AZOBR_RS21565 AZOBR_RS21570 AZOBR_RS21575 AZOBR_RS21580
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. -0.2 -5.6 -6.2 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.1 -5.4 -6.5 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. -0.4 -5.0 -5.1 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. -0.3 -4.3 -5.2 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. -0.2 -2.9 -6.4 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. -0.4 -5.6 -3.3 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. -0.3 -3.8 -3.8 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. -0.3 -4.6 -2.9 N.D.
stress 2-pentyl-furan 1.335528 mM N.D. -0.2 -4.0 -3.4 N.D.
stress 2-pentyl-furan 1.780666667 mM N.D. +0.3 -4.0 -3.3 N.D.
stress 2-pentyl-furan 1.335528 mM N.D. +0.0 -3.9 -3.2 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.1 -4.6 -2.4 N.D.
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days N.D. -0.2 -2.8 -3.7 N.D.
stress 2-pentyl-furan 1.780666667 mM N.D. +0.2 -3.1 -3.4 N.D.
stress 2-pentyl-furan 1.780666667 mM N.D. +0.5 -2.9 -3.3 N.D.
lb LB N.D. -0.1 +0.2 -5.5 N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. -0.4 -1.4 -3.0 N.D.
stress 2-pentyl-furan 1.335528 mM N.D. +0.1 -3.2 -1.6 N.D.
motility outer cut, LB soft agar motility assay N.D. -1.6 -1.9 -1.0 N.D.
nitrogen source Parabanic Acid (N) N.D. +0.3 -2.0 -2.4 N.D.
nitrogen source casaminos (N) N.D. +0.2 -0.2 -3.6 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.1 +0.9 -4.5 N.D.
stress 3-octanone 6.3476 mM N.D. -0.3 -2.0 -0.9 N.D.
stress 3-octanone 6.3476 mM N.D. +0.4 -0.5 -3.0 N.D.
stress 2-pentyl-furan 1.335528 mM N.D. -0.1 -2.4 -0.6 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.0 +0.5 -3.1 N.D.
motility inner cut, LB soft agar motility assay N.D. -0.8 -0.9 +2.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.2 +1.7 +1.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.5 +1.0 +1.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.5 +1.6 +1.5 N.D.
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