Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS21140 and AZOBR_RS21145 are separated by 164 nucleotidesAZOBR_RS21145 and AZOBR_RS21150 are separated by 92 nucleotidesAZOBR_RS21150 and AZOBR_RS21155 are separated by 111 nucleotidesAZOBR_RS21155 and AZOBR_RS21160 are separated by 232 nucleotides AZOBR_RS21140: AZOBR_RS21140 - C4-dicarboxylate ABC transporter, at 1,523,617 to 1,524,918 _RS21140 AZOBR_RS21145: AZOBR_RS21145 - NADP-dependent malic enzyme, at 1,525,083 to 1,527,401 _RS21145 AZOBR_RS21150: AZOBR_RS21150 - N-acetylglucosaminyltransferase, at 1,527,494 to 1,529,161 _RS21150 AZOBR_RS21155: AZOBR_RS21155 - hypothetical protein, at 1,529,273 to 1,529,974 _RS21155 AZOBR_RS21160: AZOBR_RS21160 - hypothetical protein, at 1,530,207 to 1,530,782 _RS21160
Group Condition AZOBR_RS21140 AZOBR_RS21145 AZOBR_RS21150 AZOBR_RS21155 AZOBR_RS21160
carbon source succinate (C) -3.2 -3.1 -0.3 -0.8 -0.1
carbon source succinate (C) -3.1 -3.0 +0.1 -0.7 -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.6 -0.5 +0.4 -5.5 -0.2
carbon source L-Malic (C) -2.2 -3.2 -0.0 -0.6 -0.0
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -1.1 -0.9 +0.4 -4.0 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.6 -1.0 +0.4 -4.0 -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.6 -0.8 +0.2 -3.9 -0.4
stress 2-pentyl-furan 1.780666667 mM -1.7 -0.8 -0.2 -2.4 -0.1
carbon source L-Malic (C) -1.9 -3.1 +0.4 -0.7 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -1.0 -0.2 -0.1 -3.8 -0.0
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.6 +0.3 +0.4 -4.9 -0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.8 +0.6 -0.3 -4.1 -0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -1.1 -0.0 +0.3 -4.2 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.7 -0.6 +0.7 -3.6 -0.5
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.1 +0.1 +0.4 -4.6 +0.1
stress 2-pentyl-furan 1.335528 mM -0.2 -0.9 -0.0 -2.3 +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.2 -2.9 +0.3 -0.3 -0.1
stress 2-pentyl-furan 1.335528 mM +0.4 -0.3 -0.4 -2.9 -0.1
stress Sisomicin 0.01 mg/ml -0.1 -2.7 +0.3 +0.0 -0.6
stress 3-octanone 6.3476 mM -1.3 -0.4 -0.0 -0.5 -0.8
stress Benzethonium chloride 0.003 mM -0.6 -1.8 -0.5 +0.1 +0.1
stress 2-pentyl-furan 1.780666667 mM +0.3 -0.7 +0.2 -2.4 +0.5
motility outer cut, LB soft agar motility assay +1.0 -0.6 +0.3 -2.5 -0.2
motility inner cut, LB soft agar motility assay +0.1 -0.4 -0.5 -1.6 +0.3
stress 2-pentyl-furan 1.335528 mM +0.5 -1.4 -0.3 -0.5 -0.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.1 -2.3 +0.3 +0.1 -0.1
stress Paraquat dichloride 0.04 mg/ml +0.8 -1.1 -0.6 -0.4 -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.3 -2.0 +0.4 -0.1 +0.3
stress 2-pentyl-furan 1.780666667 mM +0.5 -1.2 +0.1 -0.4 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -0.9 +0.1 +1.0 +0.4
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