Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS20945 and AZOBR_RS20950 are separated by 514 nucleotidesAZOBR_RS20950 and AZOBR_RS20955 are separated by 35 nucleotidesAZOBR_RS20955 and AZOBR_RS20960 are separated by 107 nucleotidesAZOBR_RS20960 and AZOBR_RS20965 are separated by 18 nucleotides AZOBR_RS20945: AZOBR_RS20945 - transposase, at 1,456,416 to 1,456,787 _RS20945 AZOBR_RS20950: AZOBR_RS20950 - membrane protein, at 1,457,302 to 1,458,453 _RS20950 AZOBR_RS20955: AZOBR_RS20955 - hypothetical protein, at 1,458,489 to 1,459,658 _RS20955 AZOBR_RS20960: AZOBR_RS20960 - hypothetical protein, at 1,459,766 to 1,460,365 _RS20960 AZOBR_RS20965: AZOBR_RS20965 - alcohol dehydrogenase, at 1,460,384 to 1,461,559 _RS20965
Group Condition AZOBR_RS20945 AZOBR_RS20950 AZOBR_RS20955 AZOBR_RS20960 AZOBR_RS20965
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.3 -0.4 -0.9 -0.3
carbon source a-Ketoglutaric (C) N.D. -0.4 +0.0 -0.6 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.0 +0.2 -1.0 -0.1
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. -0.4 -0.3 -0.2 -0.2
stress 3-octanone 6.3476 mM N.D. -0.2 -0.1 -0.5 -0.1
carbon source D,L-Lactate (C) N.D. -0.2 -0.2 -0.5 -0.1
nitrogen source casaminos (N) N.D. -0.3 -0.2 -0.0 -0.4
stress 2-pentyl-furan 2.671 mM N.D. -0.8 -0.2 +0.1 +0.0
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.1 -0.5 -0.1 -0.4
stress D-Cycloserine 0.125 mg/ml N.D. -0.3 -0.3 -0.3 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.2 +0.1 -0.6 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 +0.1 -0.7 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.0 +0.0 -0.8 +0.2
lb LB N.D. +0.0 -0.0 -0.8 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.2 +0.1 -0.7 -0.1
stress Sisomicin 0.01 mg/ml N.D. -0.1 +0.1 -0.8 +0.5
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM N.D. +0.1 +0.0 -0.6 +0.2
nitrogen source D-Alanine (N) N.D. -0.2 -0.4 +0.4 -0.0
carbon source butyrate (C) N.D. -0.2 -0.3 +0.4 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.2 -0.3 +0.2 -0.3
stress 2-pentyl-furan 1.780666667 mM N.D. -0.2 -0.1 -0.1 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.0 -0.1 +0.5 -0.4
stress 2-Furfuraldehyde 0.2 vol% N.D. -0.1 +0.2 +0.3 -0.4
carbon source Glycerol (C) N.D. +0.0 -0.3 +0.4 +0.3
stress 2-pentyl-furan 1.780666667 mM N.D. -0.5 +0.3 +0.3 +0.3
stress Paraquat dichloride 0.04 mg/ml N.D. +0.4 +0.2 -0.3 +0.2
stress 2-pentyl-furan 1.335528 mM N.D. -0.0 +0.3 +0.3 +0.2
stress 2-pentyl-furan 1.780666667 mM N.D. -0.1 +0.7 +0.2 +0.1
motility outer cut, LB soft agar motility assay N.D. +0.3 -0.0 +0.6 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.3 +0.0 +0.9 +0.5
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