Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS20630 and AZOBR_RS20635 are separated by 37 nucleotidesAZOBR_RS20635 and AZOBR_RS20640 are separated by 265 nucleotidesAZOBR_RS20640 and AZOBR_RS20645 overlap by 1 nucleotidesAZOBR_RS20645 and AZOBR_RS20650 are separated by 21 nucleotides AZOBR_RS20630: AZOBR_RS20630 - acetyltransferase, at 1,388,808 to 1,389,293 _RS20630 AZOBR_RS20635: AZOBR_RS20635 - putative methyl-accepting chemotaxis protein, at 1,389,331 to 1,391,007 _RS20635 AZOBR_RS20640: AZOBR_RS20640 - glutamyl-tRNA amidotransferase subunit C, at 1,391,273 to 1,391,560 _RS20640 AZOBR_RS20645: AZOBR_RS20645 - glutamyl-tRNA(Gln) amidotransferase, at 1,391,560 to 1,393,041 _RS20645 AZOBR_RS20650: AZOBR_RS20650 - glutamyl-tRNA amidotransferase subunit B, at 1,393,063 to 1,394,514 _RS20650
Group Condition AZOBR_RS20630 AZOBR_RS20635 AZOBR_RS20640 AZOBR_RS20645 AZOBR_RS20650
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.5 -0.0 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.1 -0.0 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.1 -0.1 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 +0.1 N.D. N.D. N.D.
stress D-Cycloserine 0.125 mg/ml -0.4 -0.3 N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay -0.7 -0.1 N.D. N.D. N.D.
nitrogen source L-Leucine (N) -0.5 -0.3 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -0.1 N.D. N.D. N.D.
stress Chlorite 1 mM -0.4 -0.2 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 +0.2 N.D. N.D. N.D.
nitrogen source Urea (N) -0.6 +0.1 N.D. N.D. N.D.
motility inner cut, LB soft agar motility assay -0.6 +0.1 N.D. N.D. N.D.
lb LB -0.5 +0.2 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 +0.4 N.D. N.D. N.D.
stress 2-pentyl-furan 1.335528 mM -0.3 +0.4 N.D. N.D. N.D.
stress 2-pentyl-furan 2.671 mM -0.3 +0.5 N.D. N.D. N.D.
carbon source L-Glutamine (C) +0.4 -0.2 N.D. N.D. N.D.
stress 3-octanone 6.3476 mM +0.5 -0.2 N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.5 -0.2 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.6 -0.3 N.D. N.D. N.D.
carbon source Glycerol (C) +0.6 -0.2 N.D. N.D. N.D.
carbon source L-Lactate (C) +0.5 -0.1 N.D. N.D. N.D.
nitrogen source L-Threonine (N) +0.6 -0.2 N.D. N.D. N.D.
lb no stress control LB no stress control +0.6 -0.1 N.D. N.D. N.D.
carbon source succinate (C) +0.6 -0.0 N.D. N.D. N.D.
anaerobic; rich media LB; anaerobic +0.7 -0.1 N.D. N.D. N.D.
nitrogen source L-Glutamic (N) +0.5 +0.2 N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.7 +0.0 N.D. N.D. N.D.
stress 2-pentyl-furan 1.780666667 mM +0.4 +0.3 N.D. N.D. N.D.
nitrogen source L-Isoleucine (N) +0.6 +0.3 N.D. N.D. N.D.
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