Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS20500 and AZOBR_RS20505 are separated by 95 nucleotidesAZOBR_RS20505 and AZOBR_RS20510 are separated by 234 nucleotidesAZOBR_RS20510 and AZOBR_RS20515 are separated by 230 nucleotidesAZOBR_RS20515 and AZOBR_RS20525 are separated by 2409 nucleotides AZOBR_RS20500: AZOBR_RS20500 - transcriptional regulator, at 1,364,889 to 1,365,437 _RS20500 AZOBR_RS20505: AZOBR_RS20505 - LysR family transcriptional regulator, at 1,365,533 to 1,366,417 _RS20505 AZOBR_RS20510: AZOBR_RS20510 - alkyl hydroperoxide reductase subunit C, at 1,366,652 to 1,367,215 _RS20510 AZOBR_RS20515: AZOBR_RS20515 - alkyl hydroperoxide reductase subunit F, at 1,367,446 to 1,369,026 _RS20515 AZOBR_RS20525: AZOBR_RS20525 - FAD-binding molybdopterin dehydrogenase, at 1,371,436 to 1,372,434 _RS20525
Group Condition AZOBR_RS20500 AZOBR_RS20505 AZOBR_RS20510 AZOBR_RS20515 AZOBR_RS20525
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.9 N.D. +1.1 -1.8 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.9 N.D. +0.8 -0.7 -0.3
carbon source D-Gluconic Acid (C) -2.3 N.D. -1.3 +0.5 +0.0
carbon source L-Lactate (C) -0.5 N.D. -2.0 -0.1 -0.2
nitrogen source L-Serine (N) -1.0 N.D. -1.6 +0.2 +0.1
carbon source L-Malic (C) -0.5 N.D. -1.8 +0.2 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 N.D. +0.7 -1.4 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 N.D. +0.9 -1.4 -0.1
stress copper (II) chloride 2 mM +0.5 N.D. +0.8 -1.8 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.9 N.D. +0.6 -1.5 -0.2
motility inner cut, LB soft agar motility assay -0.9 N.D. +3.1 -1.1 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 N.D. +1.2 -1.2 +0.5
motility inner cut, LB soft agar motility assay -1.9 N.D. +3.6 -0.1 -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 N.D. +2.0 -1.0 +0.0
stress 3-octanone 6.3476 mM -0.8 N.D. +1.3 +0.4 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.2 N.D. +2.7 -0.6 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.3 N.D. +2.5 -0.9 +0.4
motility inner cut, LB soft agar motility assay -0.1 N.D. +3.4 -1.0 -0.3
stress Cobalt chloride 0.3 mM -0.7 N.D. +2.8 -0.0 +0.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.1 N.D. +2.7 -0.5 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.1 N.D. +3.1 -0.5 +0.1
stress Paraquat dichloride 0.02 mg/ml +0.3 N.D. +4.2 -1.1 -0.5
lb LB -1.3 N.D. +4.8 -0.2 -0.0
stress Cobalt chloride 0.2 mM -0.4 N.D. +3.5 +0.4 -0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.6 N.D. +2.8 -0.2 +0.2
stress Paraquat dichloride 0.04 mg/ml +0.4 N.D. +4.3 -0.6 -0.7
stress Sisomicin 0.01 mg/ml -1.5 N.D. +5.2 -0.1 -0.1
lb LB -0.9 N.D. +4.9 -0.2 +0.1
stress D-Cycloserine 0.125 mg/ml +0.3 N.D. +4.1 -0.4 +0.2
stress Chlorite 1 mM -0.1 N.D. +4.6 -0.3 +0.1
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