Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS20360 and AZOBR_RS20365 are separated by 68 nucleotidesAZOBR_RS20365 and AZOBR_RS20370 are separated by 237 nucleotidesAZOBR_RS20370 and AZOBR_RS20375 are separated by 205 nucleotidesAZOBR_RS20375 and AZOBR_RS20380 are separated by 179 nucleotides AZOBR_RS20360: AZOBR_RS20360 - hypothetical protein, at 1,337,101 to 1,337,607 _RS20360 AZOBR_RS20365: AZOBR_RS20365 - hydrogenase expression/formation protein HupF, at 1,337,676 to 1,337,948 _RS20365 AZOBR_RS20370: AZOBR_RS20370 - protein hupE, at 1,338,186 to 1,338,788 _RS20370 AZOBR_RS20375: AZOBR_RS20375 - beta-lactamase, at 1,338,994 to 1,339,989 _RS20375 AZOBR_RS20380: AZOBR_RS20380 - beta-lactamase, at 1,340,169 to 1,341,173 _RS20380
Group Condition AZOBR_RS20360 AZOBR_RS20365 AZOBR_RS20370 AZOBR_RS20375 AZOBR_RS20380
nitrogen source Urea (N) +0.6 N.D. -5.5 -0.4 +0.2
nitrogen source Parabanic Acid (N) +0.3 N.D. -4.9 -0.1 +0.1
nitrogen source Parabanic Acid (N) +0.2 N.D. -4.0 -0.2 -0.3
nitrogen source Urea (N) +0.5 N.D. -4.5 -0.5 +0.3
stress 3-octanone 6.3476 mM -1.4 N.D. -0.6 +0.2 -0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -2.0 N.D. +0.3 -0.2 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -1.7 N.D. -0.5 +0.4 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.6 N.D. -0.9 -0.3 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.9 N.D. -0.1 +0.1 -0.7
stress Cobalt chloride 0.2 mM -0.0 N.D. -0.9 -0.2 -0.4
nitrogen source L-Citrulline (N) +0.2 N.D. -1.6 -0.1 +0.1
stress 2-pentyl-furan 1.780666667 mM +0.2 N.D. -1.2 +0.2 -0.6
nitrogen source L-Citrulline (N) +0.0 N.D. -1.7 +0.0 +0.3
motility inner cut, LB soft agar motility assay -1.2 N.D. +0.6 -0.5 -0.1
stress 3-octanone 6.3476 mM -1.1 N.D. +0.5 +0.1 -0.4
stress 1-octen-3-ol 0.6784116667 mM +0.3 N.D. -0.6 -0.2 -0.5
stress 1-octen-3-ol 0.6784116667 mM +0.4 N.D. -0.7 -0.1 -0.4
nitrogen source L-Isoleucine (N) +0.7 N.D. -1.0 +0.3 -0.7
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -1.2 N.D. +0.5 +0.1 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. +0.5 +0.2 -1.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -1.3 N.D. +0.7 +0.1 +0.2
stress Benzethonium chloride 0.003 mM -1.7 N.D. +0.4 +0.3 +0.5
stress Sisomicin 0.01 mg/ml -0.6 N.D. +0.7 -0.1 -0.4
nitrogen source casaminos (N) +0.4 N.D. -0.7 +0.1 -0.2
stress D-Cycloserine 0.125 mg/ml +1.0 N.D. -0.8 +0.6 -1.0
nitrogen source L-Lysine (N) +0.6 N.D. -0.7 -0.3 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.3 N.D. -0.6 +0.6 +0.7
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.5 N.D. -0.3 +0.0 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 N.D. +0.7 +0.4 -0.6
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.6 N.D. +0.7 +0.3 +0.5
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