Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS20290 and AZOBR_RS20295 are separated by 133 nucleotidesAZOBR_RS20295 and AZOBR_RS20300 are separated by 90 nucleotidesAZOBR_RS20300 and AZOBR_RS20305 are separated by 107 nucleotidesAZOBR_RS20305 and AZOBR_RS20310 are separated by 169 nucleotides AZOBR_RS20290: AZOBR_RS20290 - dihydroneopterin aldolase, at 1,322,866 to 1,323,249 _RS20290 AZOBR_RS20295: AZOBR_RS20295 - modification methylase, at 1,323,383 to 1,324,468 _RS20295 AZOBR_RS20300: AZOBR_RS20300 - ribonuclease HII, at 1,324,559 to 1,325,173 _RS20300 AZOBR_RS20305: AZOBR_RS20305 - cAMP-binding protein, at 1,325,281 to 1,326,003 _RS20305 AZOBR_RS20310: AZOBR_RS20310 - hypothetical protein, at 1,326,173 to 1,326,670 _RS20310
Group Condition AZOBR_RS20290 AZOBR_RS20295 AZOBR_RS20300 AZOBR_RS20305 AZOBR_RS20310
nitrogen source casaminos (N) N.D. N.D. -3.5 -0.0 +0.0
motility inner cut, LB soft agar motility assay N.D. N.D. -3.1 -0.2 +0.3
lb LB N.D. N.D. -3.3 -0.2 +0.7
stress 3-octanone 6.3476 mM N.D. N.D. -1.8 -0.3 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -2.2 +0.1 -0.5
nitrogen source casaminos (N) N.D. N.D. -2.0 -0.1 +0.2
stress 2-pentyl-furan 1.780666667 mM N.D. N.D. -1.8 +0.1 -0.1
stress 2-pentyl-furan 2.671 mM N.D. N.D. -1.7 -0.1 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -2.0 -0.1 +0.4
stress 2-pentyl-furan 1.780666667 mM N.D. N.D. -1.7 +0.1 -0.1
stress 1-octen-3-ol 0.7753276667 mM N.D. N.D. -1.7 -0.1 +0.1
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM N.D. N.D. -1.7 -0.2 +0.2
nitrogen source D-Alanine (N) N.D. N.D. -1.6 -0.1 +0.1
stress 2-pentyl-furan 1.335528 mM N.D. N.D. -1.6 -0.1 +0.1
motility inner cut, LB soft agar motility assay N.D. N.D. -1.7 -0.2 +0.5
stress 1-octen-3-ol 0.7753276667 mM N.D. N.D. -1.7 -0.2 +0.4
nitrogen source L-Glutamic (N) N.D. N.D. -1.3 +0.3 -0.3
carbon source D-Gluconic Acid (C) N.D. N.D. -0.7 -0.6 +0.3
lb LB N.D. N.D. -1.2 -0.1 +0.3
nitrogen source L-Threonine (N) N.D. N.D. -1.2 +0.5 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. +0.6 -0.4 +0.6
stress Paraquat dichloride 0.02 mg/ml N.D. N.D. +0.9 -0.4 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. +1.1 -0.3 +0.5
lb LB N.D. N.D. +1.6 -0.1 -0.1
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. N.D. +1.2 +0.2 +0.2
carbon source pyruvate (C) N.D. N.D. +1.1 +0.2 +0.3
motility inner cut, LB soft agar motility assay N.D. N.D. +2.3 -0.6 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +1.7 +0.3 -0.0
nitrogen source L-Histidine (N) N.D. N.D. +1.8 +0.5 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +2.7 -0.0 -0.4
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