Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS20270 and AZOBR_RS20275 are separated by 128 nucleotidesAZOBR_RS20275 and AZOBR_RS20280 are separated by 38 nucleotidesAZOBR_RS20280 and AZOBR_RS20285 are separated by 1397 nucleotidesAZOBR_RS20285 and AZOBR_RS20290 are separated by 79 nucleotides AZOBR_RS20270: AZOBR_RS20270 - hypothetical protein, at 1,317,690 to 1,318,169 _RS20270 AZOBR_RS20275: AZOBR_RS20275 - adenine glycosylase, at 1,318,298 to 1,319,380 _RS20275 AZOBR_RS20280: AZOBR_RS20280 - hypothetical protein, at 1,319,419 to 1,320,180 _RS20280 AZOBR_RS20285: AZOBR_RS20285 - hypothetical protein, at 1,321,578 to 1,322,786 _RS20285 AZOBR_RS20290: AZOBR_RS20290 - dihydroneopterin aldolase, at 1,322,866 to 1,323,249 _RS20290
Group Condition AZOBR_RS20270 AZOBR_RS20275 AZOBR_RS20280 AZOBR_RS20285 AZOBR_RS20290
carbon source L-Malic (C) N.D. -0.4 -0.3 -0.2 N.D.
nitrogen source nitrate (N) N.D. -0.6 -0.3 -0.0 N.D.
motility outer cut, LB soft agar motility assay N.D. +0.2 -0.3 -0.7 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.7 -0.9 -0.3 N.D.
stress 2-pentyl-furan 1.780666667 mM N.D. -0.6 -0.1 +0.3 N.D.
nitrogen source L-Isoleucine (N) N.D. -0.7 +0.4 +0.1 N.D.
stress Thallium(I) acetate 0.008 mg/ml N.D. -0.4 +0.5 -0.3 N.D.
nitrogen source L-Leucine (N) N.D. -0.5 +0.4 +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.2 -0.4 +0.3 N.D.
stress 3-octanone 6.3476 mM N.D. -0.4 +0.3 +0.2 N.D.
stress copper (II) chloride 2 mM N.D. -0.4 +0.3 +0.3 N.D.
stress 2-Furfuraldehyde 0.2 vol% N.D. +0.8 -0.5 +0.2 N.D.
stress Benzethonium chloride 0.003 mM N.D. +0.6 +0.4 -0.5 N.D.
motility inner cut, LB soft agar motility assay N.D. +0.2 +0.8 -0.3 N.D.
carbon source Glycerol (C) N.D. -0.2 +0.7 +0.1 N.D.
carbon source D-Lactate (C) N.D. +0.3 +0.4 +0.2 N.D.
nitrogen source L-Threonine (N) N.D. +0.5 +0.3 +0.1 N.D.
carbon source pyruvate (C) N.D. +0.3 +0.3 +0.3 N.D.
nitrogen source D-Alanine (N) N.D. +0.3 +0.5 +0.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.6 +0.5 -0.1 N.D.
lb LB N.D. +0.3 +0.6 +0.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.5 +0.5 +0.1 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.2 +1.0 -0.1 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.0 +0.9 +0.3 N.D.
carbon source L-Lactate (C) N.D. +0.2 +0.6 +0.4 N.D.
nitrogen source Glucuronamide (N) N.D. +0.3 +0.4 +0.5 N.D.
stress Sisomicin 0.01 mg/ml N.D. +0.4 +0.2 +0.6 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.4 +0.6 +0.3 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.3 +0.7 +0.3 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.1 +0.9 +0.4 N.D.
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