Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS20235 and AZOBR_RS20240 are separated by 91 nucleotidesAZOBR_RS20240 and AZOBR_RS20245 are separated by 163 nucleotidesAZOBR_RS20245 and AZOBR_RS20250 are separated by 160 nucleotidesAZOBR_RS20250 and AZOBR_RS20260 are separated by 1284 nucleotides AZOBR_RS20235: AZOBR_RS20235 - transcriptional regulator, at 1,309,463 to 1,310,107 _RS20235 AZOBR_RS20240: AZOBR_RS20240 - histidine kinase, at 1,310,199 to 1,310,633 _RS20240 AZOBR_RS20245: AZOBR_RS20245 - membrane protein, at 1,310,797 to 1,311,495 _RS20245 AZOBR_RS20250: AZOBR_RS20250 - hypothetical protein, at 1,311,656 to 1,312,129 _RS20250 AZOBR_RS20260: AZOBR_RS20260 - chromosome segregation ATPase, at 1,313,414 to 1,316,875 _RS20260
Group Condition AZOBR_RS20235 AZOBR_RS20240 AZOBR_RS20245 AZOBR_RS20250 AZOBR_RS20260
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -1.3 N.D. -1.6 -0.5 -0.3
stress D-Cycloserine 0.125 mg/ml -1.7 N.D. -0.1 -0.5 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.3 N.D. -0.0 -0.1 -0.7
stress Chlorite 1 mM -1.2 N.D. +0.0 -1.1 -0.7
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -1.2 N.D. -2.2 +0.0 +0.6
motility inner cut, LB soft agar motility assay -0.7 N.D. -0.8 +0.3 -1.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -1.4 N.D. -1.8 -0.2 +0.9
stress Cobalt chloride 0.3 mM -2.0 N.D. -0.0 -0.0 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.9 N.D. +0.3 -0.7 -0.9
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -1.0 N.D. -0.9 -0.2 -0.0
lb LB -1.1 N.D. -0.4 -0.5 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.0 N.D. -0.1 +0.3 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.8 N.D. -1.5 -0.0 +0.4
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.9 N.D. -1.2 -0.2 +0.4
stress Cobalt chloride 0.2 mM -0.8 N.D. -0.6 +0.2 -0.6
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.8 N.D. -1.3 -0.1 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 N.D. +0.4 +0.0 -1.1
stress Vancomycin 0.03 mg/ml -0.9 N.D. +0.3 +0.1 -0.8
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.5 N.D. -0.9 +0.2 +0.6
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.5 N.D. -0.8 +0.1 +0.6
carbon source D-Gluconic Acid (C) +0.8 N.D. -0.7 -0.3 -0.4
nitrogen source L-Histidine (N) -0.6 N.D. -0.0 -0.6 +0.9
nitrogen source L-Isoleucine (N) +2.3 N.D. -0.4 -1.4 -0.0
carbon source a-Ketoglutaric (C) +1.8 N.D. +0.1 -0.5 -0.4
motility outer cut, LB soft agar motility assay +1.8 N.D. -0.7 +0.4 -0.1
carbon source Glycerol (C) +2.3 N.D. +0.1 -0.2 -0.5
carbon source D-Gluconic Acid (C) +1.4 N.D. +0.1 -0.2 +0.4
carbon source a-Ketoglutaric (C) +2.1 N.D. +0.1 -0.1 -0.3
carbon source Glycerol (C) +1.8 N.D. +0.0 -0.2 +0.6
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +2.1 N.D. +0.1 +0.1 +0.3
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