Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS19575 and AZOBR_RS19580 are separated by 379 nucleotidesAZOBR_RS19580 and AZOBR_RS19585 are separated by 172 nucleotidesAZOBR_RS19585 and AZOBR_RS19590 are separated by 27 nucleotidesAZOBR_RS19590 and AZOBR_RS19595 are separated by 245 nucleotides AZOBR_RS19575: AZOBR_RS19575 - chromosome partitioning protein, at 1,162,885 to 1,163,535 _RS19575 AZOBR_RS19580: AZOBR_RS19580 - AsnC family transcriptional regulator, at 1,163,915 to 1,164,343 _RS19580 AZOBR_RS19585: AZOBR_RS19585 - arginase, at 1,164,516 to 1,165,463 _RS19585 AZOBR_RS19590: AZOBR_RS19590 - ornithine-oxoacid aminotransferase, at 1,165,491 to 1,166,708 _RS19590 AZOBR_RS19595: AZOBR_RS19595 - deacylase, at 1,166,954 to 1,168,069 _RS19595
Group Condition AZOBR_RS19575 AZOBR_RS19580 AZOBR_RS19585 AZOBR_RS19590 AZOBR_RS19595
nitrogen source L-Citrulline (N) N.D. -3.0 N.D. -2.0 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -3.1 N.D. -0.4 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -2.0 N.D. -0.3 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.6 N.D. -0.4 -0.8
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -1.5 N.D. -0.8 -0.5
nitrogen source L-Citrulline (N) N.D. -1.8 N.D. -0.4 -0.1
nitrogen source L-Glutamic (N) N.D. -0.7 N.D. -0.5 -0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -1.5 N.D. +0.3 -0.8
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM N.D. -1.1 N.D. -0.4 -0.2
stress 1-octen-3-ol 0.7753276667 mM N.D. -1.2 N.D. -0.2 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.1 N.D. -0.9 -0.8
stress 1-octen-3-ol 0.6784116667 mM N.D. -0.9 N.D. -0.4 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 N.D. -0.5 -0.7
carbon source D-Gluconic Acid (C) N.D. -0.9 N.D. -0.3 -0.1
anaerobic; rich media LB; anaerobic N.D. -0.5 N.D. -0.7 +0.2
nitrogen source L-Serine (N) N.D. -0.9 N.D. -0.2 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. -0.4 N.D. -0.7 +0.4
stress 3-octanone 5.2896 mM N.D. -1.1 N.D. +0.3 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. -0.5 N.D. -0.6 +0.6
stress 1-octen-3-ol 0.6784116667 mM N.D. -0.8 N.D. -0.0 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.7 N.D. -0.1 -0.9
nitrogen source L-Isoleucine (N) N.D. +1.2 N.D. -1.1 -0.3
nitrogen source L-Aspartic Acid (N) N.D. +0.9 N.D. -0.6 +0.1
motility inner cut, LB soft agar motility assay N.D. +0.9 N.D. -0.2 -0.2
stress 2-pentyl-furan 2.671 mM N.D. +0.8 N.D. -0.8 +0.9
stress 3-octanone 6.3476 mM N.D. -0.1 N.D. +0.6 +0.7
stress 3-octanone 6.3476 mM N.D. +0.7 N.D. +0.8 -0.2
motility outer cut, LB soft agar motility assay N.D. +0.6 N.D. -0.6 +1.4
stress Chlorite 1 mM N.D. +1.0 N.D. +0.7 +0.4
motility inner cut, LB soft agar motility assay N.D. +0.7 N.D. +1.4 +0.4
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