Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS19335 and AZOBR_RS19340 are separated by 55 nucleotidesAZOBR_RS19340 and AZOBR_RS19345 are separated by 64 nucleotidesAZOBR_RS19345 and AZOBR_RS19350 are separated by 579 nucleotidesAZOBR_RS19350 and AZOBR_RS19355 are separated by 129 nucleotides AZOBR_RS19335: AZOBR_RS19335 - asparagine synthase, at 1,106,554 to 1,108,506 _RS19335 AZOBR_RS19340: AZOBR_RS19340 - long-chain fatty acid--CoA ligase, at 1,108,562 to 1,110,121 _RS19340 AZOBR_RS19345: AZOBR_RS19345 - acyl carrier protein, at 1,110,186 to 1,110,449 _RS19345 AZOBR_RS19350: AZOBR_RS19350 - polysaccharide deacetylase, at 1,111,029 to 1,111,877 _RS19350 AZOBR_RS19355: AZOBR_RS19355 - glycosyl transferase family 1, at 1,112,007 to 1,112,972 _RS19355
Group Condition AZOBR_RS19335 AZOBR_RS19340 AZOBR_RS19345 AZOBR_RS19350 AZOBR_RS19355
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.1 -0.1 N.D. -0.3 -1.0
stress Chlorite 1 mM +0.0 -0.1 N.D. -0.4 -0.7
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.1 -0.1 N.D. -0.7 -0.2
motility inner cut, LB soft agar motility assay -0.6 -0.1 N.D. +0.3 -0.5
nitrogen source L-Histidine (N) -0.4 -0.0 N.D. -0.3 +0.4
nitrogen source nitrate (N) -0.1 -0.2 N.D. +0.6 -0.3
carbon source Glycerol (C) +0.4 +0.2 N.D. +1.2 -1.6
stress 3-octanone 6.3476 mM -0.3 +0.1 N.D. +0.6 -0.1
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days +0.3 +0.3 N.D. -0.5 +0.2
stress 2-pentyl-furan 2.671 mM +0.0 -0.3 N.D. +0.2 +0.8
carbon source Glycerol (C) +0.3 +0.2 N.D. +1.5 -1.3
carbon source L-Glutamine (C) +0.1 +0.1 N.D. +0.6 +0.2
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.2 +0.1 N.D. +0.3 +0.5
nitrogen source L-Glutamic (N) +0.2 +0.2 N.D. +0.8 +0.2
carbon source succinate (C) +0.4 +0.3 N.D. +0.4 +0.5
nitrogen source Parabanic Acid (N) +0.2 +0.2 N.D. +0.8 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 +0.0 N.D. +1.8 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -0.0 N.D. +0.7 +1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 +0.2 N.D. +0.6 +1.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +0.5 N.D. +1.1 +0.4
nitrogen source Cytosine (N) +0.7 +0.6 N.D. +0.9 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.4 N.D. +1.1 +0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +0.5 N.D. +1.3 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 -0.1 N.D. +1.1 +1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 +0.3 N.D. +1.3 +1.2
carbon source a-Ketoglutaric (C) +0.8 +0.8 N.D. +0.9 +0.4
carbon source a-Ketoglutaric (C) +0.8 +0.9 N.D. +1.1 +0.3
nitrogen source L-Isoleucine (N) +0.9 +1.0 N.D. +1.0 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 +0.7 N.D. +1.2 +0.8
nitrogen source Cytosine (N) +1.0 +1.0 N.D. +1.2 +0.6
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