Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS19035 and AZOBR_RS19040 are separated by 79 nucleotidesAZOBR_RS19040 and AZOBR_RS19045 are separated by 221 nucleotidesAZOBR_RS19045 and AZOBR_RS19050 are separated by 307 nucleotidesAZOBR_RS19050 and AZOBR_RS19055 are separated by 114 nucleotides AZOBR_RS19035: AZOBR_RS19035 - molecular chaperone Hsp33, at 1,040,067 to 1,041,014 _RS19035 AZOBR_RS19040: AZOBR_RS19040 - hypothetical protein, at 1,041,094 to 1,041,681 _RS19040 AZOBR_RS19045: AZOBR_RS19045 - S-adenosyl-L-homocysteine hydrolase, at 1,041,903 to 1,043,222 _RS19045 AZOBR_RS19050: AZOBR_RS19050 - phosphoesterase, at 1,043,530 to 1,044,162 _RS19050 AZOBR_RS19055: AZOBR_RS19055 - hypothetical protein, at 1,044,277 to 1,044,789 _RS19055
Group Condition AZOBR_RS19035 AZOBR_RS19040 AZOBR_RS19045 AZOBR_RS19050 AZOBR_RS19055
nitrogen source Ammonium chloride (N) -1.2 -0.5 N.D. -0.1 N.D.
carbon source D-Lactate (C) -0.8 -0.4 N.D. -0.3 N.D.
motility inner cut, LB soft agar motility assay -1.0 -0.2 N.D. -0.2 N.D.
nitrogen source casaminos (N) -1.5 -0.0 N.D. +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.0 -0.2 N.D. +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.7 +0.1 N.D. +0.5 N.D.
carbon source butyrate (C) -0.9 -0.0 N.D. -0.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.6 N.D. -0.6 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -1.6 +0.1 N.D. +0.4 N.D.
carbon source L-Leucine (C) -0.6 -0.3 N.D. -0.1 N.D.
stress Nickel (II) chloride 0.5 mM -0.7 -0.3 N.D. +0.0 N.D.
carbon source L-Lactate (C) -0.7 +0.0 N.D. -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.9 +0.2 N.D. -0.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 +0.1 N.D. -0.2 N.D.
stress 1-octen-3-ol 0.6030326667 mM -0.7 +0.1 N.D. -0.2 N.D.
nitrogen source L-Threonine (N) -1.2 +0.3 N.D. +0.0 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -1.0 +0.2 N.D. +0.0 N.D.
stress 3-octanone 6.3476 mM -0.8 +0.3 N.D. -0.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.8 +0.1 N.D. +0.1 N.D.
carbon source Ethanol (C) -0.9 +0.1 N.D. +0.1 N.D.
stress Thallium(I) acetate 0.008 mg/ml -0.7 +0.3 N.D. -0.1 N.D.
stress 2-pentyl-furan 1.335528 mM +0.6 -0.2 N.D. -0.4 N.D.
nitrogen source L-Glutamic (N) +0.7 -0.7 N.D. +0.1 N.D.
stress Benzethonium chloride 0.003 mM +0.4 -0.5 N.D. +0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 +0.7 N.D. -0.5 N.D.
stress Paraquat dichloride 0.04 mg/ml +1.0 +0.0 N.D. -0.2 N.D.
stress 2-pentyl-furan 1.780666667 mM +0.5 +0.0 N.D. +0.5 N.D.
motility outer cut, LB soft agar motility assay +1.1 +0.3 N.D. -0.2 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +1.0 +0.2 N.D. +0.2 N.D.
motility inner cut, LB soft agar motility assay +1.0 +0.6 N.D. +0.3 N.D.
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