Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS18870 and AZOBR_RS18875 are separated by 123 nucleotidesAZOBR_RS18875 and AZOBR_RS18880 are separated by 34 nucleotidesAZOBR_RS18880 and AZOBR_RS18885 are separated by 161 nucleotidesAZOBR_RS18885 and AZOBR_RS18890 are separated by 156 nucleotides AZOBR_RS18870: AZOBR_RS18870 - RNA polymerase sigma54 factor, at 1,003,949 to 1,005,526 _RS18870 AZOBR_RS18875: AZOBR_RS18875 - ribose ABC transporter permease, at 1,005,650 to 1,006,246 _RS18875 AZOBR_RS18880: AZOBR_RS18880 - nitrogen regulatory protein, at 1,006,281 to 1,006,739 _RS18880 AZOBR_RS18885: AZOBR_RS18885 - 3-demethylubiquinone-9 3-methyltransferase, at 1,006,901 to 1,007,662 _RS18885 AZOBR_RS18890: AZOBR_RS18890 - aspartate kinase, at 1,007,819 to 1,009,054 _RS18890
Group Condition AZOBR_RS18870 AZOBR_RS18875 AZOBR_RS18880 AZOBR_RS18885 AZOBR_RS18890
stress 2-pentyl-furan 1.335528 mM -6.7 -2.0 N.D. N.D. N.D.
nitrogen source D-Alanine (N) -5.6 -2.1 N.D. N.D. N.D.
stress 2-pentyl-furan 1.780666667 mM -5.6 -2.0 N.D. N.D. N.D.
nitrogen source nitrate (N) -6.7 -0.5 N.D. N.D. N.D.
nitrogen source D-Alanine (N) -6.0 -1.2 N.D. N.D. N.D.
nitrogen source L-Threonine (N) -4.9 -2.2 N.D. N.D. N.D.
stress 2-pentyl-furan 1.780666667 mM -5.9 -0.7 N.D. N.D. N.D.
stress 2-pentyl-furan 1.335528 mM -5.3 -0.2 N.D. N.D. N.D.
nitrogen source nitrate (N) -5.4 -0.0 N.D. N.D. N.D.
stress 2-pentyl-furan 1.335528 mM -3.8 -1.6 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -5.0 -0.1 N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -5.1 +0.1 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -4.8 +0.1 N.D. N.D. N.D.
nitrogen source L-Serine (N) -3.3 -1.3 N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -4.4 -0.2 N.D. N.D. N.D.
nitrogen source Cytosine (N) -4.8 +0.4 N.D. N.D. N.D.
nitrogen source L-Threonine (N) -3.4 -1.0 N.D. N.D. N.D.
nitrogen source Cytosine (N) -4.0 -0.2 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -4.5 +0.5 N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -4.1 +0.1 N.D. N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -3.7 -0.1 N.D. N.D. N.D.
nitrogen source L-Isoleucine (N) -0.8 -2.9 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -3.8 +0.1 N.D. N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -3.7 +0.6 N.D. N.D. N.D.
stress 2-pentyl-furan 1.780666667 mM -3.7 +0.6 N.D. N.D. N.D.
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -3.5 +0.6 N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay -1.6 +3.4 N.D. N.D. N.D.
motility inner cut, LB soft agar motility assay -0.7 +3.1 N.D. N.D. N.D.
motility inner cut, LB soft agar motility assay -0.7 +3.8 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +2.2 +1.4 N.D. N.D. N.D.
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