Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS18525 and AZOBR_RS18530 are separated by 52 nucleotidesAZOBR_RS18530 and AZOBR_RS18535 are separated by 78 nucleotidesAZOBR_RS18535 and AZOBR_RS18540 are separated by 102 nucleotidesAZOBR_RS18540 and AZOBR_RS18545 are separated by 194 nucleotides AZOBR_RS18525: AZOBR_RS18525 - uracil-DNA glycosylase, at 925,968 to 926,504 _RS18525 AZOBR_RS18530: AZOBR_RS18530 - hypothetical protein, at 926,557 to 927,273 _RS18530 AZOBR_RS18535: AZOBR_RS18535 - LysR family transcriptional regulator, at 927,352 to 928,260 _RS18535 AZOBR_RS18540: AZOBR_RS18540 - endoribonuclease L-PSP, at 928,363 to 928,761 _RS18540 AZOBR_RS18545: AZOBR_RS18545 - putative two-component response regulator:antitermination factor NasT, at 928,956 to 929,573 _RS18545
Group Condition AZOBR_RS18525 AZOBR_RS18530 AZOBR_RS18535 AZOBR_RS18540 AZOBR_RS18545
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.9 -0.8 -0.3 N.D. -2.0
nitrogen source nitrate (N) +0.2 +0.1 -0.1 N.D. -4.6
nitrogen source nitrate (N) +0.4 -0.4 +0.1 N.D. -3.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 +0.3 +0.1 N.D. -3.9
motility inner cut, LB soft agar motility assay -0.8 -0.1 -0.1 N.D. -1.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.1 -1.7 -1.0 N.D. -0.8
stress Paraquat dichloride 0.02 mg/ml -1.0 -0.2 -0.2 N.D. -0.7
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.9 -0.4 +0.1 N.D. -0.4
stress Chlorite 1 mM -0.5 +0.0 -0.4 N.D. -0.7
stress nitrite 10 mM -0.0 +0.0 -0.3 N.D. -1.2
nitrogen source L-Phenylalanine (N) +0.5 -0.2 -0.1 N.D. -1.6
stress 3-octanone 5.2896 mM -0.6 -0.4 +0.1 N.D. -0.4
nitrogen source L-Citrulline (N) -0.0 +0.2 +0.1 N.D. -1.4
lb LB -0.9 +0.0 +0.2 N.D. -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 -0.8 +0.1 N.D. -1.0
carbon source D-Gluconic Acid (C) +0.4 -0.0 -0.1 N.D. -1.1
nitrogen source D-Alanine (N) +0.5 +0.0 -0.1 N.D. -1.3
nitrogen source L-Threonine (N) -0.2 -0.4 -0.4 N.D. +0.5
motility outer cut, LB soft agar motility assay -2.0 +0.4 +0.2 N.D. +0.8
stress 2-pentyl-furan 2.671 mM +0.0 -0.6 -0.5 N.D. +0.6
stress D-Cycloserine 0.125 mg/ml -0.3 -0.5 -0.2 N.D. +0.9
nitrogen source L-Isoleucine (N) +0.9 -0.4 +0.8 N.D. -1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.8 -0.2 +0.2 N.D. +1.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.1 -0.7 +0.6 N.D. -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 -0.1 +0.5 N.D. -0.4
lb LB +0.2 +0.4 -0.3 N.D. +1.0
nitrogen source casaminos (N) -0.2 +0.2 +0.4 N.D. +1.1
nitrogen source L-Serine (N) +0.1 +0.2 -0.1 N.D. +1.4
nitrogen source casaminos (N) +0.6 +0.0 +0.4 N.D. +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 +0.0 -0.1 N.D. +2.3
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