Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS18520 and AZOBR_RS18525 are separated by 40 nucleotidesAZOBR_RS18525 and AZOBR_RS18530 are separated by 52 nucleotidesAZOBR_RS18530 and AZOBR_RS18535 are separated by 78 nucleotidesAZOBR_RS18535 and AZOBR_RS18540 are separated by 102 nucleotides AZOBR_RS18520: AZOBR_RS18520 - chromosome partitioning protein, at 925,046 to 925,927 _RS18520 AZOBR_RS18525: AZOBR_RS18525 - uracil-DNA glycosylase, at 925,968 to 926,504 _RS18525 AZOBR_RS18530: AZOBR_RS18530 - hypothetical protein, at 926,557 to 927,273 _RS18530 AZOBR_RS18535: AZOBR_RS18535 - LysR family transcriptional regulator, at 927,352 to 928,260 _RS18535 AZOBR_RS18540: AZOBR_RS18540 - endoribonuclease L-PSP, at 928,363 to 928,761 _RS18540
Group Condition AZOBR_RS18520 AZOBR_RS18525 AZOBR_RS18530 AZOBR_RS18535 AZOBR_RS18540
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -1.9 -0.8 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +1.1 -1.7 -1.0 N.D.
motility outer cut, LB soft agar motility assay N.D. -2.0 +0.4 +0.2 N.D.
stress Paraquat dichloride 0.02 mg/ml N.D. -1.0 -0.2 -0.2 N.D.
stress 2-pentyl-furan 1.780666667 mM N.D. -0.3 -0.6 -0.3 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.9 -0.4 +0.1 N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. +0.0 -0.7 -0.5 N.D.
nitrogen source L-Histidine (N) N.D. -0.4 -0.5 -0.2 N.D.
stress 2-pentyl-furan 2.671 mM N.D. +0.0 -0.6 -0.5 N.D.
nitrogen source L-Threonine (N) N.D. -0.2 -0.4 -0.4 N.D.
motility inner cut, LB soft agar motility assay N.D. -0.8 -0.1 -0.1 N.D.
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM N.D. -0.5 -0.4 +0.0 N.D.
stress 3-octanone 5.2896 mM N.D. -0.6 -0.4 +0.1 N.D.
stress Chlorite 1 mM N.D. -0.5 +0.0 -0.4 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.8 -0.2 +0.2 N.D.
lb LB N.D. -0.9 +0.0 +0.2 N.D.
motility inner cut, LB soft agar motility assay N.D. -0.2 -0.5 +0.4 N.D.
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM N.D. -0.3 -0.2 +0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.5 -0.8 +0.1 N.D.
carbon source succinate (C) N.D. +0.5 -0.3 -0.1 N.D.
stress Sisomicin 0.01 mg/ml N.D. +0.2 -0.4 +0.4 N.D.
stress 3-octanone 6.3476 mM N.D. +0.5 -0.0 +0.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.6 +0.3 +0.1 N.D.
motility inner cut, LB soft agar motility assay N.D. +0.4 +0.6 +0.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +1.1 -0.7 +0.6 N.D.
nitrogen source casaminos (N) N.D. +0.6 +0.0 +0.4 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.5 +0.5 +0.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.8 -0.1 +0.5 N.D.
carbon source butyrate (C) N.D. +0.6 +0.3 +0.4 N.D.
nitrogen source L-Isoleucine (N) N.D. +0.9 -0.4 +0.8 N.D.
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