Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS18510 and AZOBR_RS18515 are separated by 1308 nucleotidesAZOBR_RS18515 and AZOBR_RS18520 are separated by 40 nucleotidesAZOBR_RS18520 and AZOBR_RS18525 are separated by 40 nucleotidesAZOBR_RS18525 and AZOBR_RS18530 are separated by 52 nucleotides AZOBR_RS18510: AZOBR_RS18510 - plasmid replication protein, at 922,015 to 922,944 _RS18510 AZOBR_RS18515: AZOBR_RS18515 - chromosome partitioning protein, at 924,253 to 925,005 _RS18515 AZOBR_RS18520: AZOBR_RS18520 - chromosome partitioning protein, at 925,046 to 925,927 _RS18520 AZOBR_RS18525: AZOBR_RS18525 - uracil-DNA glycosylase, at 925,968 to 926,504 _RS18525 AZOBR_RS18530: AZOBR_RS18530 - hypothetical protein, at 926,557 to 927,273 _RS18530
Group Condition AZOBR_RS18510 AZOBR_RS18515 AZOBR_RS18520 AZOBR_RS18525 AZOBR_RS18530
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. -1.9 -0.8
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. -2.0 +0.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. N.D. N.D. -0.9 -0.4
stress Paraquat dichloride 0.02 mg/ml N.D. N.D. N.D. -1.0 -0.2
stress 3-octanone 5.2896 mM N.D. N.D. N.D. -0.6 -0.4
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM N.D. N.D. N.D. -0.5 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. N.D. -0.8 -0.2
motility inner cut, LB soft agar motility assay N.D. N.D. N.D. -0.8 -0.1
stress 2-pentyl-furan 1.780666667 mM N.D. N.D. N.D. -0.3 -0.6
nitrogen source L-Histidine (N) N.D. N.D. N.D. -0.4 -0.5
lb LB N.D. N.D. N.D. -0.9 +0.0
stress D-Cycloserine 0.125 mg/ml N.D. N.D. N.D. -0.3 -0.5
lb LB N.D. N.D. N.D. -0.6 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. +1.1 -1.7
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. N.D. N.D. +0.0 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. +0.5 -0.8
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM N.D. N.D. N.D. +0.3 -0.5
nitrogen source Parabanic Acid (N) N.D. N.D. N.D. +0.4 -0.4
nitrogen source nitrate (N) N.D. N.D. N.D. +0.4 -0.4
stress 3-octanone 5.2896 mM N.D. N.D. N.D. +0.4 -0.3
carbon source succinate (C) N.D. N.D. N.D. +0.5 -0.3
dmso control DMSO control N.D. N.D. N.D. +0.5 -0.3
nitrogen source Glucuronamide (N) N.D. N.D. N.D. +0.5 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. +1.1 -0.7
nitrogen source L-Isoleucine (N) N.D. N.D. N.D. +0.9 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. +0.8 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. +0.6 +0.3
carbon source butyrate (C) N.D. N.D. N.D. +0.6 +0.3
motility inner cut, LB soft agar motility assay N.D. N.D. N.D. +0.4 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. N.D. +0.5 +0.5
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