Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS18185 and AZOBR_RS18190 are separated by 400 nucleotidesAZOBR_RS18190 and AZOBR_RS18195 overlap by 4 nucleotidesAZOBR_RS18195 and AZOBR_RS18200 are separated by 100 nucleotidesAZOBR_RS18200 and AZOBR_RS18205 are separated by 230 nucleotides AZOBR_RS18185: AZOBR_RS18185 - sugar ABC transporter, at 838,037 to 839,191 _RS18185 AZOBR_RS18190: AZOBR_RS18190 - hypothetical protein, at 839,592 to 840,293 _RS18190 AZOBR_RS18195: AZOBR_RS18195 - hypothetical protein, at 840,290 to 843,593 _RS18195 AZOBR_RS18200: AZOBR_RS18200 - hypothetical protein, at 843,694 to 846,412 _RS18200 AZOBR_RS18205: AZOBR_RS18205 - outer membrane efflux protein, at 846,643 to 847,959 _RS18205
Group Condition AZOBR_RS18185 AZOBR_RS18190 AZOBR_RS18195 AZOBR_RS18200 AZOBR_RS18205
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 N.D. -0.5 -0.1 N.D.
stress Paraquat dichloride 0.04 mg/ml -0.2 N.D. -0.2 -0.5 N.D.
stress Paraquat dichloride 0.02 mg/ml -0.4 N.D. -0.3 -0.3 N.D.
stress 2-pentyl-furan 2.671 mM -0.3 N.D. -0.4 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 N.D. -0.8 +0.1 N.D.
nitrogen source L-Leucine (N) -0.2 N.D. -0.7 +0.0 N.D.
stress Sisomicin 0.01 mg/ml -0.3 N.D. -0.3 -0.2 N.D.
stress 2-pentyl-furan 1.780666667 mM +0.1 N.D. -0.1 -0.8 N.D.
nitrogen source L-Threonine (N) +0.0 N.D. -0.5 -0.2 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.0 N.D. -0.2 -0.4 N.D.
stress D-Cycloserine 0.125 mg/ml -0.3 N.D. -0.0 -0.4 N.D.
anaerobic; rich media LB; anaerobic +0.1 N.D. -0.1 -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 N.D. -0.5 +0.1 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.1 N.D. +0.0 -0.5 N.D.
lb LB -0.4 N.D. +0.2 -0.1 N.D.
carbon source D-Gluconic Acid (C) -0.5 N.D. +0.2 +0.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.0 N.D. +0.2 -0.5 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.2 N.D. +0.1 -0.4 N.D.
stress 1-octen-3-ol 0.7753276667 mM -0.3 N.D. +0.3 +0.1 N.D.
stress 2-pentyl-furan 1.335528 mM +0.1 N.D. +0.2 -0.3 N.D.
lb no stress control LB no stress control +0.2 N.D. +0.2 -0.3 N.D.
stress 3-octanone 5.2896 mM -0.0 N.D. +0.4 -0.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 N.D. -0.2 +0.2 N.D.
stress Benzethonium chloride 0.003 mM +0.3 N.D. +0.0 +0.3 N.D.
stress copper (II) chloride 2 mM +0.3 N.D. +0.1 +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 N.D. +0.3 -0.1 N.D.
motility outer cut, LB soft agar motility assay +0.0 N.D. +0.7 -0.0 N.D.
nitrogen source L-Aspartic Acid (N) +0.1 N.D. +0.2 +0.4 N.D.
stress 2-pentyl-furan 1.780666667 mM +0.2 N.D. +0.5 -0.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 N.D. +0.3 +0.6 N.D.
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