Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS18180 and AZOBR_RS18185 are separated by 60 nucleotidesAZOBR_RS18185 and AZOBR_RS18190 are separated by 400 nucleotidesAZOBR_RS18190 and AZOBR_RS18195 overlap by 4 nucleotidesAZOBR_RS18195 and AZOBR_RS18200 are separated by 100 nucleotides AZOBR_RS18180: AZOBR_RS18180 - sugar ABC transporter, at 837,155 to 837,976 _RS18180 AZOBR_RS18185: AZOBR_RS18185 - sugar ABC transporter, at 838,037 to 839,191 _RS18185 AZOBR_RS18190: AZOBR_RS18190 - hypothetical protein, at 839,592 to 840,293 _RS18190 AZOBR_RS18195: AZOBR_RS18195 - hypothetical protein, at 840,290 to 843,593 _RS18195 AZOBR_RS18200: AZOBR_RS18200 - hypothetical protein, at 843,694 to 846,412 _RS18200
Group Condition AZOBR_RS18180 AZOBR_RS18185 AZOBR_RS18190 AZOBR_RS18195 AZOBR_RS18200
motility inner cut, LB soft agar motility assay -1.2 -0.3 N.D. -0.1 -0.1
stress D-Cycloserine 0.125 mg/ml -0.6 -0.3 N.D. -0.0 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.9 +0.0 N.D. +0.2 -0.5
stress Paraquat dichloride 0.02 mg/ml -0.1 -0.4 N.D. -0.3 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.6 -0.0 N.D. -0.3 -0.1
motility inner cut, LB soft agar motility assay -0.8 -0.2 N.D. -0.0 +0.1
stress 2-pentyl-furan 1.780666667 mM -0.1 +0.1 N.D. -0.1 -0.8
stress Paraquat dichloride 0.04 mg/ml +0.1 -0.2 N.D. -0.2 -0.5
nitrogen source L-Threonine (N) -0.2 +0.0 N.D. -0.5 -0.2
stress Sisomicin 0.01 mg/ml +0.1 -0.3 N.D. -0.3 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.0 N.D. -0.5 +0.1
lb LB -0.4 -0.4 N.D. +0.2 -0.1
nitrogen source L-Leucine (N) +0.2 -0.2 N.D. -0.7 +0.0
stress 2-pentyl-furan 2.671 mM +0.2 -0.3 N.D. -0.4 -0.3
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.5 +0.2 N.D. -0.1 -0.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.3 -0.0 N.D. +0.2 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 -0.2 N.D. -0.8 +0.1
nitrogen source L-Leucine (N) -0.6 +0.1 N.D. +0.1 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 -0.4 N.D. -0.5 -0.1
anaerobic; rich media LB; anaerobic +0.3 +0.1 N.D. -0.1 -0.4
stress 2-pentyl-furan 1.780666667 mM -0.7 +0.2 N.D. +0.5 -0.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.1 +0.2 N.D. +0.1 -0.4
stress 2-pentyl-furan 1.335528 mM +0.3 +0.1 N.D. +0.2 -0.3
motility outer cut, LB soft agar motility assay -0.2 +0.0 N.D. +0.7 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 +0.2 N.D. -0.4 +0.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.7 -0.1 N.D. +0.0 +0.1
stress 3-octanone 6.3476 mM +0.3 +0.2 N.D. -0.0 +0.3
stress Benzethonium chloride 0.003 mM +0.3 +0.3 N.D. +0.0 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 +0.4 N.D. +0.3 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 +0.2 N.D. +0.3 +0.6
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