Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS18170 and AZOBR_RS18175 are separated by 257 nucleotidesAZOBR_RS18175 and AZOBR_RS18180 are separated by 4 nucleotidesAZOBR_RS18180 and AZOBR_RS18185 are separated by 60 nucleotidesAZOBR_RS18185 and AZOBR_RS18190 are separated by 400 nucleotides AZOBR_RS18170: AZOBR_RS18170 - sugar ABC transporter, at 834,770 to 836,005 _RS18170 AZOBR_RS18175: AZOBR_RS18175 - sugar ABC transporter, at 836,263 to 837,150 _RS18175 AZOBR_RS18180: AZOBR_RS18180 - sugar ABC transporter, at 837,155 to 837,976 _RS18180 AZOBR_RS18185: AZOBR_RS18185 - sugar ABC transporter, at 838,037 to 839,191 _RS18185 AZOBR_RS18190: AZOBR_RS18190 - hypothetical protein, at 839,592 to 840,293 _RS18190
Group Condition AZOBR_RS18170 AZOBR_RS18175 AZOBR_RS18180 AZOBR_RS18185 AZOBR_RS18190
motility inner cut, LB soft agar motility assay -0.6 -0.2 -1.2 -0.3 N.D.
motility outer cut, LB soft agar motility assay -0.3 -1.0 -0.2 +0.0 N.D.
motility inner cut, LB soft agar motility assay -0.0 -0.3 -0.8 -0.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.1 -0.4 -0.6 -0.0 N.D.
nitrogen source L-Isoleucine (N) -0.7 -0.1 -0.2 +0.0 N.D.
carbon source D-Gluconic Acid (C) -0.4 +0.0 -0.1 -0.5 N.D.
stress 2-pentyl-furan 1.780666667 mM -0.5 -0.0 -0.7 +0.2 N.D.
lb LB -0.2 +0.0 -0.4 -0.4 N.D.
nitrogen source L-Aspartic Acid (N) -0.3 -0.2 -0.5 +0.0 N.D.
stress Paraquat dichloride 0.02 mg/ml -0.2 -0.1 -0.1 -0.4 N.D.
nitrogen source L-Leucine (N) -0.1 -0.2 -0.6 +0.1 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -1.3 +0.8 -0.4 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.0 -0.4 -0.5 +0.2 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.0 -0.2 -0.4 +0.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.3 +0.1 -0.9 +0.0 N.D.
stress D-Cycloserine 0.125 mg/ml +0.4 +0.1 -0.6 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.5 +0.2 +0.1 N.D.
stress 2-pentyl-furan 2.671 mM -0.2 +0.1 +0.2 -0.3 N.D.
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM -0.2 +0.2 -0.2 +0.3 N.D.
stress 2-pentyl-furan 1.780666667 mM -0.2 -0.4 +0.3 +0.4 N.D.
stress benzoic 0.29 mg/ml +0.2 +0.2 -0.4 +0.1 N.D.
stress Benzethonium chloride 0.003 mM -0.5 +0.1 +0.3 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.5 +0.4 +0.2 N.D.
dmso control DMSO control -0.2 +0.4 -0.1 +0.2 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.1 -0.3 +0.7 -0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +0.2 +0.4 -0.2 N.D.
nitrogen source L-Glutamic (N) -0.2 +0.3 +0.3 +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.1 +0.8 +0.2 N.D.
stress 3-octanone 6.3476 mM +0.3 +0.6 +0.5 -0.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.3 +0.5 +0.4 N.D.
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