Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS18135 and AZOBR_RS18145 are separated by 1167 nucleotidesAZOBR_RS18145 and AZOBR_RS18150 overlap by 19 nucleotidesAZOBR_RS18150 and AZOBR_RS18155 are separated by 61 nucleotidesAZOBR_RS18155 and AZOBR_RS18160 are separated by 70 nucleotides AZOBR_RS18135: AZOBR_RS18135 - LacI family transcription regulator, at 826,379 to 827,440 _RS18135 AZOBR_RS18145: AZOBR_RS18145 - racemase, at 828,608 to 829,855 _RS18145 AZOBR_RS18150: AZOBR_RS18150 - membrane protein, at 829,837 to 830,988 _RS18150 AZOBR_RS18155: AZOBR_RS18155 - enoyl-CoA hydratase, at 831,050 to 831,796 _RS18155 AZOBR_RS18160: AZOBR_RS18160 - fumarylacetoacetate hydrolase, at 831,867 to 833,012 _RS18160
Group Condition AZOBR_RS18135 AZOBR_RS18145 AZOBR_RS18150 AZOBR_RS18155 AZOBR_RS18160
motility outer cut, LB soft agar motility assay -4.2 +0.1 -0.8 +1.1 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -3.0 +0.3 -0.3 -0.1 -0.6
nitrogen source L-Phenylalanine (N) -1.9 +0.1 -0.1 -0.1 -1.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.1 -0.2 +0.4 +0.3 -1.8
carbon source D-Gluconic Acid (C) -4.3 +0.0 -0.1 +1.1 +0.2
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM -0.0 -0.0 +0.1 -0.3 -2.6
nitrogen source L-Isoleucine (N) -2.5 +0.0 -0.3 +0.3 -0.5
nitrogen source L-Citrulline (N) -1.9 -0.1 -0.2 -0.3 -0.4
stress benzoic 0.29 mg/ml -1.4 -0.0 -0.4 -0.1 -0.7
carbon source butyrate (C) -2.4 +0.1 +0.1 -0.2 -0.1
stress 2-pentyl-furan 1.335528 mM -1.4 -0.1 +0.3 -0.6 -0.4
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM -0.4 -0.2 +0.2 -0.4 -1.3
stress 3-octanone 4.2317 mM +0.3 -0.1 -0.1 -0.5 -1.8
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.6 -0.2 +0.6 +0.0 -0.5
nitrogen source L-Threonine (N) -1.7 +0.1 +0.4 +0.3 -0.9
lb LB -1.9 -0.0 -0.6 -0.0 +0.9
nitrogen source L-Glutamic (N) -2.4 +0.4 -0.3 +0.1 +0.7
motility inner cut, LB soft agar motility assay -1.5 +0.1 -0.4 +0.3 +0.3
stress 3-octanone 6.3476 mM +0.0 +0.0 -0.6 -1.2 +0.7
nitrogen source L-Histidine (N) -0.2 +0.3 +0.4 +0.1 -1.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.4 +0.6 +0.8 -1.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -1.1 -0.3 +0.7 +0.7 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.3 +0.0 +0.4 +1.2 -0.8
stress Cobalt chloride 0.2 mM +1.1 +0.0 -0.6 +0.1 -0.8
stress Paraquat dichloride 0.02 mg/ml -1.0 +0.2 +0.3 -0.1 +0.9
motility inner cut, LB soft agar motility assay -0.6 +0.0 +0.1 -0.5 +1.6
stress 2-Furfuraldehyde 0.2 vol% +0.4 +0.3 -0.6 -0.3 +1.0
carbon source Glycerol (C) +1.6 +0.2 +0.2 +0.4 -0.7
carbon source a-Ketoglutaric (C) +3.8 -0.4 +0.1 +0.2 -0.3
carbon source a-Ketoglutaric (C) +3.6 +0.1 +0.0 -0.1 -0.0
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