Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS17000 and AZOBR_RS17005 are separated by 298 nucleotidesAZOBR_RS17005 and AZOBR_RS17010 overlap by 60 nucleotidesAZOBR_RS17010 and AZOBR_RS17015 are separated by 169 nucleotidesAZOBR_RS17015 and AZOBR_RS17020 are separated by 9 nucleotides AZOBR_RS17000: AZOBR_RS17000 - 1-deoxy-D-xylulose-5-phosphate synthase, at 568,540 to 570,471 _RS17000 AZOBR_RS17005: AZOBR_RS17005 - radical SAM protein, at 570,770 to 571,897 _RS17005 AZOBR_RS17010: AZOBR_RS17010 - hypothetical protein, at 571,838 to 572,107 _RS17010 AZOBR_RS17015: AZOBR_RS17015 - hypothetical protein, at 572,277 to 572,663 _RS17015 AZOBR_RS17020: AZOBR_RS17020 - hypothetical protein, at 572,673 to 573,068 _RS17020
Group Condition AZOBR_RS17000 AZOBR_RS17005 AZOBR_RS17010 AZOBR_RS17015 AZOBR_RS17020
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.3 -0.2 N.D. -3.5 +0.3
stress D-Cycloserine 0.125 mg/ml -0.2 +0.1 N.D. -3.5 +0.6
carbon source D-Gluconic Acid (C) -0.1 -0.0 N.D. -3.3 +0.7
stress 2-pentyl-furan 2.671 mM -0.4 +0.3 N.D. -2.4 +0.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.1 -0.1 N.D. -2.7 +0.7
stress Cobalt chloride 0.2 mM -0.5 -0.6 N.D. -0.4 -0.6
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.1 -0.1 N.D. -1.6 -0.3
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -0.4 +0.1 N.D. -1.9 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.2 -0.1 N.D. -1.6 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.2 N.D. -1.4 +0.2
stress 2-pentyl-furan 1.335528 mM +0.5 +0.0 N.D. -2.1 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.3 +0.1 N.D. -1.3 +0.4
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.2 +0.0 N.D. -1.5 +0.8
anaerobic; rich media LB; anaerobic -0.5 -0.0 N.D. -0.9 +0.7
nitrogen source Parabanic Acid (N) +0.1 +0.1 N.D. -1.2 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.3 +0.1 N.D. -1.4 +0.8
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.2 +0.4 N.D. -1.8 +0.9
carbon source succinate (C) +0.0 +0.4 N.D. -1.1 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.1 +0.1 N.D. -1.4 +1.1
stress 3-octanone 6.3476 mM +0.1 -0.2 N.D. +1.0 -0.4
stress 3-octanone 6.3476 mM -0.1 -0.6 N.D. +0.6 +0.8
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.1 +0.4 N.D. +1.0 -0.3
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.2 +0.4 N.D. -0.6 +1.3
carbon source L-Glutamine (C) -0.1 +0.4 N.D. +1.3 +0.0
nitrogen source D-Alanine (N) +0.0 +0.3 N.D. +0.9 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 +0.1 N.D. +0.9 +0.9
nitrogen source L-Histidine (N) -0.1 +0.5 N.D. +1.7 -0.2
nitrogen source D-Alanine (N) +0.2 +0.3 N.D. +1.0 +0.5
nitrogen source Cytosine (N) +0.2 +0.3 N.D. +1.2 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 +0.2 N.D. +2.9 +0.2
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