Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS16730 and AZOBR_RS16735 are separated by 31 nucleotidesAZOBR_RS16735 and AZOBR_RS16740 overlap by 4 nucleotidesAZOBR_RS16740 and AZOBR_RS16745 are separated by 10 nucleotidesAZOBR_RS16745 and AZOBR_RS16750 are separated by 73 nucleotides AZOBR_RS16730: AZOBR_RS16730 - uroporphyrin-III methyltransferase, at 510,694 to 511,437 _RS16730 AZOBR_RS16735: AZOBR_RS16735 - cobyrinic acid a c-diamide synthase, at 511,469 to 512,800 _RS16735 AZOBR_RS16740: AZOBR_RS16740 - cob(II)yrinic acid a c-diamide reductase, at 512,797 to 513,456 _RS16740 AZOBR_RS16745: AZOBR_RS16745 - oxidoreductase, at 513,467 to 514,180 _RS16745 AZOBR_RS16750: AZOBR_RS16750 - TetR family transcriptional regulator, at 514,254 to 514,853 _RS16750
Group Condition AZOBR_RS16730 AZOBR_RS16735 AZOBR_RS16740 AZOBR_RS16745 AZOBR_RS16750
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.1 -0.3 -0.2 +0.4 -2.9
carbon source D-Gluconic Acid (C) -0.7 +0.5 -0.2 +0.1 -3.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 -0.6 -0.6 -0.3 -0.8
motility inner cut, LB soft agar motility assay -0.6 -0.1 -0.8 -1.0 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 +0.5 +0.0 +0.3 -3.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 -0.1 +0.4 -0.4 -2.2
stress 2-pentyl-furan 1.335528 mM -0.3 -0.4 -0.6 -0.6 -0.9
motility outer cut, LB soft agar motility assay -1.0 -0.5 +0.4 -1.5 -0.1
stress 3-octanone 6.3476 mM +0.1 -0.2 +0.3 +0.1 -2.8
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.4 -1.1 +0.2 -0.1 -0.9
carbon source a-Ketoglutaric (C) -0.6 -0.7 -0.0 -0.2 -0.6
stress 2-pentyl-furan 1.780666667 mM -0.3 +0.4 -0.4 -0.6 -1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 +0.1 +0.1 -0.3 -1.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -0.2 +0.0 -0.2 -1.1
motility inner cut, LB soft agar motility assay -0.1 +0.0 -0.4 -0.8 -0.7
nitrogen source casaminos (N) -0.2 -0.5 -0.4 -0.4 -0.5
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.8 -1.2 -0.1 +0.1 +0.0
lb LB -1.2 +0.2 -0.3 -0.1 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.0 +0.2 +0.6 -2.7
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -0.8 -1.0 +0.4 +0.1 -0.3
nitrogen source L-Asparagine (N) -0.7 -0.3 +0.3 +0.1 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.3 -0.2 +0.3 -1.0
nitrogen source Ammonium chloride (N) -0.8 -0.5 +0.1 +0.5 -0.4
lb LB -0.8 +0.4 -0.3 +0.3 -0.2
nitrogen source L-Glutamic (N) +0.3 -0.3 +0.3 +0.2 -1.0
stress 2-pentyl-furan 2.671 mM -0.9 +0.2 +0.0 -0.3 +1.0
nitrogen source Cytosine (N) -0.5 -0.4 -0.1 +0.4 +0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.3 +0.5 +0.1 -0.3 +0.9
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.2 +0.7 +0.4 -0.5 +0.8
stress D-Cycloserine 0.125 mg/ml +0.2 +1.1 +0.3 +0.1 +0.9
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