Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS16670 and AZOBR_RS14370 are separated by 289 nucleotidesAZOBR_RS14370 and AZOBR_RS16675 are separated by 38 nucleotidesAZOBR_RS16675 and AZOBR_RS16680 are separated by 156 nucleotidesAZOBR_RS16680 and AZOBR_RS16685 overlap by 4 nucleotides AZOBR_RS16670: AZOBR_RS16670 - amino acid ABC transporter ATP-binding protein, at 496,623 to 497,399 _RS16670 AZOBR_RS14370: AZOBR_RS14370 - cobalamin, at 497,689 to 497,905 _RS14370 AZOBR_RS16675: AZOBR_RS16675 - CobW, at 497,944 to 499,011 _RS16675 AZOBR_RS16680: AZOBR_RS16680 - cobaltochelatase, at 499,168 to 502,581 _RS16680 AZOBR_RS16685: AZOBR_RS16685 - precorrin-3B synthase, at 502,578 to 503,984 _RS16685
Group Condition AZOBR_RS16670 AZOBR_RS14370 AZOBR_RS16675 AZOBR_RS16680 AZOBR_RS16685
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.2 N.D. -0.3 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. -0.3 N.D. -1.1 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. -0.1 N.D. -1.2 N.D.
stress Paraquat dichloride 0.02 mg/ml N.D. -1.0 N.D. -0.1 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.1 N.D. -1.1 N.D.
nitrogen source casaminos (N) N.D. -0.8 N.D. -0.1 N.D.
carbon source a-Ketoglutaric (C) N.D. -0.7 N.D. -0.2 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. -0.2 N.D. -0.6 N.D.
stress Chlorite 1 mM N.D. -0.5 N.D. -0.4 N.D.
stress Sisomicin 0.01 mg/ml N.D. -0.3 N.D. -0.5 N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. +0.0 N.D. -0.9 N.D.
nitrogen source L-Lysine (N) N.D. -0.7 N.D. -0.1 N.D.
stress 2-pentyl-furan 1.335528 mM N.D. -0.3 N.D. -0.5 N.D.
stress 3-octanone 5.2896 mM N.D. +0.1 N.D. -0.8 N.D.
nitrogen source L-Phenylalanine (N) N.D. -0.8 N.D. +0.2 N.D.
nitrogen source L-Aspartic Acid (N) N.D. -0.8 N.D. +0.3 N.D.
lb no stress control LB no stress control N.D. +0.2 N.D. -0.6 N.D.
stress nitrite 10 mM N.D. -0.6 N.D. +0.2 N.D.
nitrogen source L-Serine (N) N.D. -0.6 N.D. +0.2 N.D.
nitrogen source L-Isoleucine (N) N.D. -0.8 N.D. +0.5 N.D.
stress 2-pentyl-furan 2.671 mM N.D. +0.8 N.D. -1.0 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.6 N.D. -0.8 N.D.
stress 2-pentyl-furan 1.780666667 mM N.D. +0.6 N.D. -0.8 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.5 N.D. +0.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.8 N.D. -0.1 N.D.
stress 2-pentyl-furan 1.780666667 mM N.D. +0.4 N.D. +0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.6 N.D. +0.7 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.7 N.D. +0.6 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.7 N.D. +0.6 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.3 N.D. +1.0 N.D.
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