Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS16205 and AZOBR_RS16210 are separated by 163 nucleotidesAZOBR_RS16210 and AZOBR_RS16215 are separated by 112 nucleotidesAZOBR_RS16215 and AZOBR_RS16220 are separated by 166 nucleotidesAZOBR_RS16220 and AZOBR_RS16225 are separated by 264 nucleotides AZOBR_RS16205: AZOBR_RS16205 - hypothetical protein, at 385,020 to 385,910 _RS16205 AZOBR_RS16210: AZOBR_RS16210 - hypothetical protein, at 386,074 to 386,292 _RS16210 AZOBR_RS16215: AZOBR_RS16215 - aspartyl-tRNA synthetase, at 386,405 to 388,234 _RS16215 AZOBR_RS16220: AZOBR_RS16220 - ribonuclease D, at 388,401 to 389,567 _RS16220 AZOBR_RS16225: AZOBR_RS16225 - hypothetical protein, at 389,832 to 391,121 _RS16225
Group Condition AZOBR_RS16205 AZOBR_RS16210 AZOBR_RS16215 AZOBR_RS16220 AZOBR_RS16225
carbon source D-Gluconic Acid (C) -2.2 -0.8 N.D. -1.1 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -2.5 -0.8 N.D. -1.0 +0.0
nitrogen source L-Threonine (N) -2.9 -0.2 N.D. -0.2 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -3.5 +0.4 N.D. -0.1 +0.0
nitrogen source L-Glutamic (N) -0.9 +0.1 N.D. -2.3 +0.2
nitrogen source casaminos (N) -2.1 -0.2 N.D. -0.1 -0.4
stress 2-pentyl-furan 2.671 mM -1.9 -0.2 N.D. -0.3 -0.5
stress Paraquat dichloride 0.04 mg/ml -1.8 +0.4 N.D. -1.3 +0.2
stress Benzethonium chloride 0.003 mM -1.2 -0.9 N.D. -0.1 -0.1
stress 2-pentyl-furan 1.335528 mM -1.0 -0.2 N.D. -0.6 -0.3
nitrogen source L-Isoleucine (N) -0.8 -0.5 N.D. -0.7 -0.1
nitrogen source Glucuronamide (N) -1.6 +0.0 N.D. -0.3 -0.1
carbon source L-Leucine (C) -1.2 -0.0 N.D. -0.5 -0.2
stress Paraquat dichloride 0.02 mg/ml -1.0 -0.1 N.D. -0.5 -0.3
nitrogen source Cytosine (N) -1.4 +0.2 N.D. -0.5 -0.2
nitrogen source D-Alanine (N) -1.6 +0.1 N.D. -0.2 -0.3
stress 2-pentyl-furan 1.780666667 mM -1.5 +0.3 N.D. -0.1 -0.5
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -1.0 +0.7 N.D. -0.7 -0.7
nitrogen source L-Leucine (N) -0.6 +0.2 N.D. -0.9 -0.3
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -1.1 +0.3 N.D. +0.4 -0.2
nitrogen source L-Leucine (N) -0.8 +0.2 N.D. +0.7 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 +0.5 N.D. -1.0 +0.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.7 +0.3 N.D. +0.9 -0.5
motility inner cut, LB soft agar motility assay -0.5 -0.4 N.D. +1.2 +0.3
motility inner cut, LB soft agar motility assay +0.4 -0.5 N.D. +0.3 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 +0.8 N.D. +0.1 +0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 +0.6 N.D. -0.4 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.4 +0.1 N.D. -0.1 +1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.3 +0.2 N.D. +0.8 +0.6
motility outer cut, LB soft agar motility assay +0.3 +0.2 N.D. +0.6 +1.9
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