Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS15690 and AZOBR_RS15695 are separated by 25 nucleotidesAZOBR_RS15695 and AZOBR_RS15705 are separated by 1094 nucleotidesAZOBR_RS15705 and AZOBR_RS15715 are separated by 1851 nucleotidesAZOBR_RS15715 and AZOBR_RS15720 are separated by 235 nucleotides AZOBR_RS15690: AZOBR_RS15690 - glutamine ABC transporter ATP-binding protein, at 272,990 to 273,709 _RS15690 AZOBR_RS15695: AZOBR_RS15695 - dihydrofolate reductase, at 273,735 to 274,673 _RS15695 AZOBR_RS15705: AZOBR_RS15705 - 2-keto-4-pentenoate hydratase, at 275,768 to 276,532 _RS15705 AZOBR_RS15715: AZOBR_RS15715 - gluconokinase, at 278,384 to 278,869 _RS15715 AZOBR_RS15720: AZOBR_RS15720 - LacI family transcription regulator, at 279,105 to 280,112 _RS15720
Group Condition AZOBR_RS15690 AZOBR_RS15695 AZOBR_RS15705 AZOBR_RS15715 AZOBR_RS15720
motility inner cut, LB soft agar motility assay N.D. N.D. -2.3 N.D. N.D.
stress Chlorite 1 mM N.D. N.D. -1.3 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. -1.3 N.D. N.D.
nitrogen source L-Threonine (N) N.D. N.D. -1.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -1.1 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -1.1 N.D. N.D.
nitrogen source L-Isoleucine (N) N.D. N.D. -1.0 N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. N.D. -0.9 N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. N.D. -0.9 N.D. N.D.
lb LB N.D. N.D. -0.8 N.D. N.D.
stress 2-pentyl-furan 1.335528 mM N.D. N.D. -0.8 N.D. N.D.
anaerobic; rich media LB; anaerobic N.D. N.D. -0.6 N.D. N.D.
stress Nickel (II) chloride 0.5 mM N.D. N.D. -0.6 N.D. N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. N.D. -0.6 N.D. N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. N.D. -0.6 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.6 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.6 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.6 N.D. N.D.
lb LB N.D. N.D. -0.5 N.D. N.D.
nitrogen source Cytosine (N) N.D. N.D. +0.5 N.D. N.D.
nitrogen source L-Phenylalanine (N) N.D. N.D. +0.5 N.D. N.D.
stress D-Cycloserine 0.125 mg/ml N.D. N.D. +0.6 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. N.D. +0.6 N.D. N.D.
nitrogen source Ammonium chloride (N) N.D. N.D. +0.6 N.D. N.D.
nitrogen source L-Leucine (N) N.D. N.D. +0.6 N.D. N.D.
nitrogen source L-Aspartic Acid (N) N.D. N.D. +0.6 N.D. N.D.
motility outer cut, LB soft agar motility assay N.D. N.D. +0.6 N.D. N.D.
carbon source Glycerol (C) N.D. N.D. +0.7 N.D. N.D.
carbon source Glycerol (C) N.D. N.D. +0.7 N.D. N.D.
carbon source D-Gluconic Acid (C) N.D. N.D. +0.8 N.D. N.D.
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