Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS15680 and AZOBR_RS15685 are separated by 35 nucleotidesAZOBR_RS15685 and AZOBR_RS15690 are separated by 211 nucleotidesAZOBR_RS15690 and AZOBR_RS15695 are separated by 25 nucleotidesAZOBR_RS15695 and AZOBR_RS15705 are separated by 1094 nucleotides AZOBR_RS15680: AZOBR_RS15680 - amino acid ABC transporter permease, at 271,292 to 271,942 _RS15680 AZOBR_RS15685: AZOBR_RS15685 - ABC transporter substrate-binding protein, at 271,978 to 272,778 _RS15685 AZOBR_RS15690: AZOBR_RS15690 - glutamine ABC transporter ATP-binding protein, at 272,990 to 273,709 _RS15690 AZOBR_RS15695: AZOBR_RS15695 - dihydrofolate reductase, at 273,735 to 274,673 _RS15695 AZOBR_RS15705: AZOBR_RS15705 - 2-keto-4-pentenoate hydratase, at 275,768 to 276,532 _RS15705
Group Condition AZOBR_RS15680 AZOBR_RS15685 AZOBR_RS15690 AZOBR_RS15695 AZOBR_RS15705
nitrogen source L-Threonine (N) -1.2 +0.1 N.D. N.D. -1.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.3 -0.5 N.D. N.D. -1.3
motility inner cut, LB soft agar motility assay +0.1 +0.1 N.D. N.D. -2.3
stress Chlorite 1 mM -0.6 +0.1 N.D. N.D. -1.3
nitrogen source L-Isoleucine (N) +0.6 -1.4 N.D. N.D. -1.0
lb LB -0.7 -0.5 N.D. N.D. -0.3
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.4 -0.6 N.D. N.D. -0.9
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.6 -0.6 N.D. N.D. -0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -1.2 +0.1 N.D. N.D. +0.3
stress Sisomicin 0.01 mg/ml +0.3 -1.1 N.D. N.D. +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 +0.6 N.D. N.D. -1.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 +0.6 N.D. N.D. -0.4
stress 2-pentyl-furan 1.335528 mM -0.9 +0.5 N.D. N.D. -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.7 -0.4 N.D. N.D. -0.6
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM +0.6 -0.4 N.D. N.D. -0.4
carbon source Glycerol (C) -0.8 -0.0 N.D. N.D. +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +0.2 N.D. N.D. -0.6
stress 2-pentyl-furan 1.335528 mM +0.3 +0.6 N.D. N.D. -0.8
nitrogen source L-Leucine (N) -0.7 +0.3 N.D. N.D. +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +1.4 N.D. N.D. -1.1
stress 6-Pentyl-2H-pyran-2-one 0.7162051667 mM +0.9 -0.0 N.D. N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.2 +0.1 N.D. N.D. -0.4
nitrogen source Parabanic Acid (N) -0.2 +0.9 N.D. N.D. +0.3
stress 3-octanone 6.3476 mM +0.9 +0.4 N.D. N.D. -0.3
carbon source Glycerol (C) +0.4 +0.3 N.D. N.D. +0.7
stress D-Cycloserine 0.125 mg/ml +0.4 +0.7 N.D. N.D. +0.6
nitrogen source Cytosine (N) +0.7 +0.4 N.D. N.D. +0.5
carbon source D-Gluconic Acid (C) +0.6 +0.4 N.D. N.D. +0.8
motility outer cut, LB soft agar motility assay +0.5 +0.6 N.D. N.D. +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +1.0 +0.2 N.D. N.D. +0.6
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